改良快速法供肝切取技术在大鼠肝移植中的应用

Application of modified rapid donor liver procurement technique in rats with liver transplantation

目的探讨能改善供肝质量的快速法供肝切取技术。方法 Sprague-Dawley大鼠60只,采用改良快速法切取供肝。观察术后1周存活率、供肝大体观和灌洗后肝脏病理形态学改变。结果共完成30次行改良快速法供肝切取的大鼠原位肝移植手术。供肝热缺血和冷缺血时间分别为0和8 min,供肝切取手术时间(24.2±1.2)min,供肝修整时间(16.8±1.5)min;受体手术时间(46.3±4.8)min,无肝期(21.6±2.3)min;手术成功率93.3%(28/30);术后1周存活率86.7%(26/30)。供肝灌注后质地柔软,色泽均匀呈土黄色,光镜下肝窦内未见红细胞。结论改良快速法供肝切取技术能够提高供肝质量和术后存活率。

Objective To study the rapid donor liver procurement technique which can improve the quality of donor liver. Methods The donor livers were procured by the modified rapid liver procurement technique from 60 Sprague-Dawley rats.The one-week survival rate,gross appearance of donor liver and the changes of pathomorphology after cold flushing were observed. Results Thirty orthotopic liver transplantations were completed by using modified rapid liver procurement technique to procure the donor livers.The time of warm ischemia and cold ischemia were 0 and 8 minutes,respectively.The time of donor liver procurement surgery and liver preparation were（24.2±1.2） and（16.8±1.5） minutes,respectively.The time of recipient surgery and anhepatic phase were（46.3±4.8） and（21.6±2.3） minutes,respectively.The operative successful rate was 93.3%（28/30）,and one-week survival rate was 86.7%（26/30）.After perfusion,the donor livers were soft,and the color was terreous;no akaryocyte in hepatic sinusoid was found under the light microscope. Conclusion The modified rapid donor liver procurement technique can improve the quality of donor liver and the survival rate after operation.