脂肪酶催化红花油水解的选择性研究

SELECTIVITY OF LIPASE CATALYZED HYDROLYSIS OF SAFLOWER OIL

在无溶剂体系中用脂肪酶Lipase LVK﹑Lipase AY 30G及Lipase F-AP15催化红花油水解,测试了水解产物中脂肪酸与酯的碘值及酯中单甘酯的含量,研究了脂肪酶对脂肪酸种类的选择性以及对甘油酯键位置的选择性.结果表明,脂肪酶Lipase LVK﹑Lipase AY30G没有位置选择性和脂肪酸选择性,而Lipase F-AP15无脂肪酸选择性,但有一定的位置选择性.

Enzymatic hydrolysis of safflower oil catalyzed by Lipase LVK﹑Lipase AY 30G and Lipase F-AP15 was investigated in a solvent-free system.The selectivity of the lipase was also examined by testing the monoglycerides content in the ester and the iodine values（IV） of the fatty acid and the ester.The experiment showed that Lipase LVK and Lipase AY 30G has no selectivity on either the fatty acid or the site of the ester bond on the acylglycero1,while Lipase F-AP15 has some selectivity on the site of the ester bond on the acylglycero1 but it has no selectivity on the fatty acid either.