丹芍化纤胶囊加食苹果对大鼠肝纤维化tsp-1及TGF-β_1的影响

Effect of Danshao Huaxian Capsule adding apple on tsp-1,TGF-β_1 of fibrosis liver of rats

目的:探讨应用丹芍化纤胶囊加食苹果对大鼠肝纤维化tsp-1、TGF-β1的影响。方法:雄性健康SD大鼠46只,采用四氯化碳(CCl4)、高脂饮食加乙醇混合因素造模成功,随机分3组,分别给予相应药物及苹果汁灌胃观察8周。实验结束后RT-PCR检测血小板反应蛋白(thrombospondin-1,tsp-1)mRNA的表达,免疫组织化学技术检测肝组织转化生长因子β1(transforming growth factor,TGF-β1)的表达。结果:RT-PCR检测在正常对照组中tsp-1 mRNA有极少量表达,与正常对照组比较,肝纤维化模型组、自然恢复组tsp-1 mRNA表达显著升高(P<0.01),治疗8周后,丹芍组tsp-1 mRNA表达下降(P<0.05),丹苹组下降更显著(P<0.01)。免疫组化结果示正常肝脏组织只见很少量TGF-β1表达,治疗8周后与自然恢复组比较,丹芍组TGF-β1表达明显下降(P<0.05),丹苹组与下降更显著(P<0.01)。结论:应用丹芍化纤胶囊加食苹果能明显改善大鼠肝功能,降低肝组织tsp-1 mRNA和TGF-β1的表达,具有明显的辅助抗肝纤维化作用。

Objective： To investigate the effects of Danshao Huaxian Capsule adding apple on the fibrosis liver of rats. Methods： All the rats excepting in the normal control group were subcutaneously given 40%CCl4 injection, high-fat diet （79.5% maizena ＋0.5% cholesterol ＋20%lard） and 70%alcohol （every other day） to induce hepatic fibrosis model for eight weeks. Then rats were randomly divided into three groups. After eight weeks, the expressions of TGF-β1 was detected respectively by immunohistochemistry. And the expression of tsp-1 mRNA was determined by Real Time Quantitive RT-PCR. Results： The results from immunohistochemlstry demonstrated that： the expression of TGF-β1 was increased in the hepatic fibrosis model and spontaneous recovery group as compared with the normal control group （P〈0.01）; while the levels of TGF-β1 in the Danshao Huaxian group and apple adding Danshao Huaxian group were decreased as compared with the hepatic fibrosis model and spontaneous recovery group. The results from Real Time Quantitive RT-PCR showed that： the expression of tsp-1 mRNA in the Danshao Huaxian group and apple adding Danshao Huaxian group were decreased as compared with the hepatic fibrosis model and spontaneous recovery group（P〈0.01）. While the expression of tsp-1 mRNA in the apple adding Danshao Huaxian group was significantly decreased （P〈0.01）. Conclusion： Apple adding Danshao Huaxian maybe play a better effect in preventing the development of hepatic fibrosis through down regulation the level of tsp-1 mRNA,TGF-β1.