益阳活血方对兔病窦综合征心阳虚血瘀证模型中医证候及血液流变学的影响

Effect of Yiyang Huoxue Recipe on TCM syndrome and hemorheology of rabbit model combined sick sinus syndrome with heart-yang deficiency and blood-stasis syndrome

目的:探讨益阳活血方对病窦综合征心阳虚血瘀证的疗效。方法:60只大耳白兔随机分为正常组、模型组、阿托品组及益阳活血高、中、低剂量组,正常组不造模,余各组用醛加压注射渗透法建立兔病窦综合征心阳虚血瘀模型,连续灌胃,分别于给药前后观测各组兔体温、呼吸频率、心率、6min游泳速率等指标,并于给药后心脏取血,测定各组家兔血液流变学不同切变率下全血黏度、血浆黏度、红细胞聚集指数等指标。结果:与模型组比较,益阳活血方治疗组给药后肛温回升、呼吸频率减慢、心率增快、游泳速率增加、血液流变学全血低切黏度、血浆黏度、红细胞聚集指数较模型组降低(P<0.01,P<0.05);且高剂量组疗效优于中、低剂量组(P<0.01,P<0.05);而阿托品组除呼吸频率减慢、心率增快、游泳速率增加、红细胞聚集指数降低(P<0.01,P<0.05)外,余指标与模型组无明显差异。结论:益阳活血方能改善病窦综合征心阳虚血瘀家兔中医证候量化指标及血液流变学指标,且高剂量组疗效更优。

Objective： To study the effect of Yiyang Huoxue Recipe on rabbit model combined sick sinus syndrome with heart-yang deficiency and blood-stasis syndrome（short as rabbit model）.Methods： The rabbit model was established by damaging the sinoatrial node with 20% formaldehyde.After the model established,the rabbits received intragastric administration for 7 days.Temperature,respiratory frequency,heart ratec,swimming speed rate in 6 min were observed before and after the administration.Whole blood viscosity on different shear rate,plasma viscosity and erythrocyte aggregation index were measured after the administration.Results： Compared with model group,the rabbits of each treatment group had higher temperature,lower respiratory frequency,higher heart rate higher swimming speed rate,lower whole blood viscosity on low shear rate,lower plasma viscosity and lower erythrocyte aggregation index after administration,and there is difference between three treatment groups and model groups（P〈0.01,P〈0.05）.The atropine group rabbits had lower respiratory frequency,higher heart ratec,higher swimming speed rate and lower erythrocyte aggregation index（P〈0.01,P〈0.05）.And the index as mentioned below has difrerence between in high dose group and in other dose groups（P〈0.01,P〈0.05）.Conclusion： Yiyang Huoxue recipe can improve quantified index of TCM symptoms and hemorheology index of rabbits model and it has better effect in high dose group.