摘要
为了探索BALB/c鼠脾脏T淋巴细胞转化试验的最佳培养条件,采用四甲基偶氮唑(MTT)比色法,对脾细胞浓度和刀豆蛋白A(ConA)的浓度两个参数进行研究。结果表明,在本次实验条件下,采用5×105个/ml细胞、2.5μg/ml的ConA可获得最大的吸光度(A)值,两个因素间存在明显的交互效应。
To explore the optimum conditions of T Lymphocyte Transformation of Spleen of BALB/c Mouse in Vitro Experiments,the concentration of spleen and ConA was studied by methyl thiazolyl tetrazolium（MTT） method.The results showed that in this experiment condition the maximum absorbance was detected at a concentraion of 5×105 /ml spleen cell and 2.5 μg/ml ConA.
出处
《中国医疗器械信息》
2011年第8期31-34,共4页
China Medical Device Information
基金
山东省自然基金(ZR2010CQ030)
关键词
T淋巴细胞体外转化试验
MTT法
BALB/C鼠
T lymphocyte transformation of spleen in vitro experiments
methyl thiazolyl tetrazolium method
BALB/c mouse
