摘要
目的:探讨胡桃醌体外抗胰腺癌细胞的机制。方法:采用MTT法检测胡桃醌对BXPC-3细胞的生长抑制作用,流式细胞仪分析胡桃醌对BXPC-3细胞周期的影响。结果:MTT法试验结果显示,胡桃醌对体外培养的人胰腺癌BXPC-3细胞具有明显的生长抑制作用,24、48h的IC50值为1.64×10-5、5.8×10-5mol/L。同时胡桃醌能随着浓度的增加,时间的延长,诱导BXPC-3细胞周期停滞于G2期和S期,呈G2期和S期阻滞作用。结论:胡桃醌对胰腺癌BXPC-3细胞有明显的生长抑制作用,并能阻止其周期,诱导BXPC-3细胞凋亡。
Objective：To investigate juglone against pancreatic cancer cells in vitro mechanism.Methods：Juglone assayon BXPC-3 cell growth inhibition by MTT,analyzed juglone on BXPC-3 cell cycle by flow cytometry.Results：MTT assay shew that juglone inhibited the growth of vitro BXPC-3 human pancreatic cancer cells significantly.The IC50 was respectively 1.64×10-5,5.8×10-5 mol/L after treatment for 24,48 h by juglone.As the concentration and time of juglone could increase,induction of BXPC-3 cell cycle arrest in G2 phase and S phase.Conclusion：Juglone significantly inhibited the growth of BXPC-3 cells,and prevent cell cycle and induction apoptosis.
出处
《中国当代医药》
2011年第10期22-23,共2页
China Modern Medicine
