G-CSF对于血管性痴呆大鼠保护作用的研究

Neuroprotective Effect of Granulocyte Colony-stimulating Factor on Vascular Dementia Rats

目的通过粒集落细胞刺激因子(G-CSF)干预,对血管性痴呆大鼠的治疗效果观察,探讨G-CSF的神经保护作用,并对其机制进行初步探讨。方法将SD大鼠随机分为2-VO模型组、粒集落细胞刺激因子干预组(G-CSF组)。2-VO法建立血管性痴呆大鼠模型,G-CSF组给予G-CSF干预。使用Morris水迷宫试验,测量大鼠学习和记忆的能力;光镜下观察大鼠海马区脑组织病理学改变;免疫组织化学染色方法检测Nestin表达情况。结果当给予集落细胞刺激因子治疗后,与未给予治疗的VD组大鼠相比,认知功能得到明显改善。结论集落细胞刺激因子是一种临床使用安全的药物,本实验中表现出神经保护作用,可能与干细胞的动员有关。

Objective To observe the effect of the granulocyte colony-stimulating factor（G-CSF）in the Treatment of vascular dementia rats,and to investigate the neuroprotective effect and mechanism of G-CSF.Methods The SD rats were divided into control group and G-CSF group.All rats were subjected to permanent occlusion of bilateral common carotid arteries.The rats of G-CSF group were subjected to G-CSF treatment.The learning and memory abilities were examined in the Morris water maze.The pathological change in hippocampus of rats was observed by light microscope.The expression of Nestin was characterized by immunohistochemical staining（IHC）.Results Compared with vascular dementia rats without treatment,the cognition of rats was significantly improved after G-CSF treatment.Conclusion In this study,the data suggested that G-CSF plays a critical function in neuro-protection,which may be concerned with the activation of Neural Stem Cell.