Survivin siRNA对卵巢癌细胞侵袭的影响

Effect of survivin siRNA on the invasive capacity of ovarian carcinoma cells

目的:探讨survivin靶向小分子干扰RNA(siRNA)对卵巢癌细胞侵袭的影响。方法:在survivin基因的编码区内根据干涉位点的设计原则,体外转录合成survivin siRNA,转染人卵巢上皮性癌细胞株SKOV3细胞,用Westernblot方法鉴定干涉位点对survivin基因蛋白的干扰效果;细胞侵袭实验(transwell)检测细胞体外侵袭能力的变化。结果:survivin siRNA能有效抑制survivin蛋白的表达,siRNA组survivin蛋白的相对表达水平分别与空白对照组、非特异性组相比,差异均有统计学意义(P<0.05)。特异性干涉组细胞的侵袭能力明显低于非特异性干涉组及空白对照组,差异均具有统计学意义(P<0.001)。结论:特异性的survivin siRNA能有效降低卵巢癌细胞survivin的表达,抑制细胞侵袭能力,这为卵巢癌的基因治疗提供了一种新策略。

Objective： To investigate the effects of RNA interference （RNAi） targeting survivin gene on the invasive capacity of SKOV3 cells. Methods： Template sequence for survivin siRNA was designed and synthesized. Lipofectamine 2000 mediated transient transfection was conducted to transmit the siRNA into SKOV3 ceils. The survivin gene expression was assessed by Western blot assays. Changes of invasive capacity of SKOV3 cells were measured by transwell. Results： Sequence specific siRNA targeting survivin down-regulated the expression of survivin protein markedly after the transfection. There was a significant difference between survivin siRNA transfected group and other groups （P〈0.05）. The invasion capacity of SKOV3 cells were decreased after trancfect survivin siRNA into the cell line. Conclusion： Survivin siRNA can inhibited cell invasion capacity of SKOV3 cells significantly. The successful application of survivin siRNA extends the list of available therapeutic modalities in the treatment of human ovarian cancer.