摘要
目的探讨乙型肝炎慢性化的免疫调控机制。方法用酶联免疫吸附实验测定慢性乙型肝炎患者(CHB)的辅助性T淋巴细胞(Th1/Th2)细胞因子IFNγ-、IL-2、IL-4和IL-10的血清浓度;同时用流式细胞术检测CHB的调节性T细胞(Treg)在外周血中的分布频率。结果与健康对照组相比,CHB组血清IL-2明显减低(P<0.05),而IL-4和IL-10均明显升高(P<0.05);CHB外周血Treg频率显著高于健康对照组(P<0.05)。结论 CHB组调节性T细胞增高,这可能是HBV感染易于慢性化的免疫机制之一。
Objective To investigate the immunopathogenesis of chronic hepatitis B virus（HBV）infection.Methods Serum T helper lymphocyte（Th1）cytokines（IFN-γ,IL-2）and Th2 cytokines（IL-4,IL-10）were measured in patients with chronic HBV infection,and the frequency of CD4＋CD25＋Foxp3＋ were investigated by flow cytometry.Results Serum IL-2 level was significantly decreased,but IL-4 or IL-10 level was significantly increased and the production of Th2 cytokines was more predominant in CHB patients as compared to healthy controls（P〈0.05）.Meanwhile,CHB patients presented a higher circulating T regulatory cell frequency than healthy controls did.Conclusion An enhanced Th2 response and higher circulating T regulatory cell frequency lies in HBV infection patients,which may be responsible for the infection chronicity.
出处
《中国临床保健杂志》
CAS
2011年第4期390-391,共2页
Chinese Journal of Clinical Healthcare
基金
安徽省卫生厅科研基金(09C247)
