修复基因XRCC1 Arg194Trp位点多态性与肝癌易感性的关系

Polymorphisms of DNA repair gene XRCC1 and susceptibility to hepatic cancer

目的:探讨DNA损伤修复基因XRCC1多态与肝癌遗传易感性的关系。方法:本研究选取了130例肝癌患者及130例性别、年龄相匹配的正常对照者进行研究,采用限制性片段长度多态性方法,比较不同基因型与肝癌发病的关系。结果:变异型等位基因XRCC1 Arg/Trp及Trp/Trp的出现率在肝癌组和对照组中分别是27.69%和10.77%,(P<0.05);而野生基因型XRCC1 Arg/Arg出现率在肝癌组和对照组中分别是72.31和89.23%,(P>0.05)。结论:XRCC1基因Arg194Trp位点单核苷酸多态在肝癌的发生过程中起着至关重要的作用。

Objective：XRCC1（X-Ray repair cross-complementing group1）gene is one of the most important DNA repair gene systems,which maintains the integrity and stability of genome and prevents carcinogenesis.In this hospital-based case control study,we evaluated the association between polymorphisms XRCC1 and the risk of hepatic cancer.Methods： We genotyped the two polymorphisms by using polymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLP） in 130 confirmed hepatic cancer patients and 130 gender,age-matched cancer as controls.Results：A significant difference of XRCC1 polymorphism was observed between cases and controls.The frequency of Arg/Trp and Trp /Trp for variant XRCC1 in cases and controls were 27.69%和10.77%,respectively,obvious higher in patients than in controls,（P0.05）.Conclusion：XRCC1 may contribute to the crucial risk of developing hepatic cancer.