PCR-焦磷酸测序法快速鉴定菌血症病原菌
摘要
世界范同内成人和儿童菌血症都是高发病率和高死亡率的疾病，每年世界范围内菌血症患者有2000万例。因病原菌不能鉴定而应用广谱抗生素或不适当抗生素治疗所导致的死亡率非常高，但欧洲每年仍有135000例患者死于菌血症，在美国更是高达215000例。
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2011年第8期739-741,共3页
Chinese Journal of Laboratory Medicine
参考文献19
-
1Zaidi AK, Thaver D, Ali SA, et al. Pathogens associated with sepsis in newborns and young infants in developing countries. Pediatr Infect Dis J,2009,28 ( suppl 1 ) : 10-18.
-
2Kumar A, Roberts D, Wood KE, et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med, 2006,34 : 1589-1596.
-
3Gerdes JS. Diagnosis and management of bacterial infections in the neonate. Pediatr Clin North Am,2004,51:939-959.
-
4Bosshard PP, Zbinden R, Abels S, et al. 16S rRNA gene sequencing versus the API 20 NE system and the VITEK 2 IDGNB card for identification of nonfermenting Gram-negative bacteria in the clinical laboratory. J Clin Mierobiol, 2006,44: 1359-1366.
-
5Kim M, Heo SR, Choi SH, et al. Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci. BMC Microbiol,2008,8:233.
-
6Peters RP, van Agtmael MA, Danner SA, et al. New developments in the diagnosis of bloodstream infections. Lancet Infect Dis ,2004,4:751-760.
-
7Qian Q, Tang YW, Kolbert CP, et al. Direct identification of bacteria from positive blood cultures by amplification and sequencing of the 16S rRNA gene : evaluation of BACTEC 9240 instrument true-positive and false-positive results. J Clin Microbiol,2001,39 :3578-3582.
-
8Haanpera M, Jalava J, Huovinen P, et al. Identification of alphahemolytic streptococci by pyrosequencing the 16S rRNA gene and by use of VITEK 2. J Clin Microbiol,2007 ,45 :762-770.
-
9Innings A, Krabbe M, Ullberg M, et al. Identification of 43 Streptococcus species by pyrosequencing analysis of the rnpB gene. J Clin Microbiol,2005,43:5983-5991.
-
10Bouza E, Sousa D, Rodreguez-Creixems M,et al Is the volume of blood cultured still a significant factor in the diagnosis of bloodstream infections? J Clin Microbio1,2007 ,45 :2765-2769.
-
1王珏,王彤,陈友华.小分子检测技术在快速检测和鉴定细菌病原体中的价值[J].中外医疗,2008,27(12):39-40.
-
2黄源芳,宋晓红,尹亚非,扎拉嘎白乙拉,姬小丽,刘小康.焦磷酸测序检测产ESBLs革兰阴性菌的SHV基因型[J].检验医学,2012,27(10):829-834. 被引量：2
-
3病原生物学检验与临床[J].中国医学文摘（检验与临床）,2007,21(5):281-292.
-
4刘蕾蕊,李爽,董莉,陈念,金柯,李军.焦磷酸测序检测81例慢性乙型肝炎hbv耐药突变株结果分析[J].中华临床感染病杂志,2012,5(2):110-112. 被引量：1
-
5杨畅,冯燕,王新卫,刘新年.成年非CF支气管扩张患者呼吸道菌群与临床测量指标间的相关性分析[J].中国卫生检验杂志,2015,25(10):1587-1590. 被引量：1
-
6陈金光.焦磷酸测序技术检测结核分枝杆菌四种药物耐药性[J].世界中医药,2015,10(A01):779-780.
-
7公衍文,任绪义,胡成进.焦磷酸测序法鉴定临床常见病原菌[J].重庆医学,2010,39(22):3009-3010. 被引量：6
-
8陈之遥,周国华.焦测序技术的研究进展[J].现代生物医学进展,2008,8(8):1573-1576. 被引量：17
-
9Hanninen S.A,Darling P.B,Sole M.J.,M.E. Keith,王亭忠.充血性心力衰竭住院患者中硫胺素缺乏症的患病率[J].世界核心医学期刊文摘（心脏病学分册）,2006,2(5):47-47.
-
10郑瑞娟,王洁,秦莲花,陆俊梅,胡忠义.焦磷酸测序技术在利福平rpoB基因突变检测中的应用评价[J].中华预防医学杂志,2008,42(2):77-80. 被引量：2
