血管紧张素Ⅱ受体-1基因A1166C多态性与脑梗死的关系

Relationship between polymorphism of angiotensin Ⅱ trpe 1 receptor gene A1166C and cerebral infarction

目的研究血管紧张素Ⅱ受体-1(AT1R)基因A1166C多态性与脑梗死的相关性。方法应用PCR及限制性片段长度多态性技术,对120例脑梗死患者(脑梗死组)和150名健康对照者(正常对照组)的AT1R基因A1166C多态位点进行检测,并比较两组间AT1R基因A1166C基因型和等位基因频率。结果经Hardy-Weinberg检验结果表明,A1166C多态位点在脑梗死组和正常对照组的频率分布具有群体代表性。脑梗死组AT1R基因A1166C位点AC基因型频率和C等位基因频率分别为35.0%和17.5%,正常对照组分别为16.0%和8.0%,两组比较差异有统计学意义(均P<0.05)。结论 AT1R基因A1166C位点的AC基因型和C等位基因可能是脑梗死患者的发病危险因素。

Objective To investigate the relationship between polymorphism of angiotensin Ⅱ type 1 receptor （AT1R） gene Al166C and cerebral infarction. Methods Frequencies of AT1R gene Al166C genotype and allele were sequenced in 120 cerebral infarction patients （CI group） and 150 normal controls （NC group） by PCR and restriction fragment length polymorphism technique, and the result were compared. Results The distributions of genotype frequency A1166C in CI group and NC group had group representation through Hardy-Weinberg analysis. Frequency of AC genotype and C allele in CI group were 35.0% , 17.5% respectively; in NC group were 16.0% , 8.0% respectively. There were significant differences between the two groups （ all P 〈 0. 05 ）. Conclusion The AC genotype and C allele of AT1R A1166C may be the risk factor of cerebral infarction.