摘要
将家蝇天蚕素(终浓度50μmol/L)加入对数生长期的人肝癌BEL-7402细胞中作用12h,扫描电镜观察天蚕素对人肝癌细胞细胞膜的影响。在此基础上,采用绿色荧光素异硫氰酸(FITC)标记天蚕素,运用激光共聚焦显微镜观察FITC标记的天蚕素与人肝癌细胞细胞膜的作用。肿瘤细胞经天蚕素作用12h后,扫描电镜显示细胞膜表面微绒毛大部分消失;激光共聚焦显微镜显示大部分天蚕素与细胞膜结合,细胞内也发现部分天蚕素进入。
Human hepatocelluar carcinoma BEL-7402 cells were treated with 50 p, mol/L Musca domestica cecropin for 12 h, and observed under scanning electron microscope. The effect of Musca domestica cecropin labeled with FITC （FITC-cecropin） on BEL-7402 cells was detected by laser scanning confocal microscopy. The scanning electron microscopy showed that most microvilli on the surface of BEL-7402 cells disappeared at 12 h after cecropin treatment. The laser scanning confocal microscopy revealed that most FITC-cecropin combined with BEL-7402 cell membrane, and partly in the cytoplasm.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2011年第4期271-273,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
广东省科技厅社会发展项目(No2010B031200011)
广州市社会发展攻关项目(No2005Z3-E0211)~~
关键词
扫描电镜
激光共聚焦显微镜
天蚕素
肿瘤细胞
细胞膜
Scanning electron microscope
Laser scanning confocal microscope
Cecropin
Tumor ceil
Membrane
