三色堇花药培养技术研究初探

Elementary Study on Anther Culture of Pansy(Viola×wittrockiana)

研究了三色堇花蕾消毒方法、花蕾低温预处理和不同培养基组分对花药愈伤组织诱导率及其分化的影响。结果表明:采用0.1%HgCl2灭菌20min是三色堇花蕾较为理想的表面灭菌时间。基因型不同花药愈伤组织的诱导率也不尽相同,而且愈伤组织的诱导率总体不高。选择合适的培养基组分、激素种类和其配比以及合适的培养条件可能是影响三色堇花药培养成功的关键因子。

The effects of different ways of bud sterilization,bud cold pretreatment,different combinations of plant growth regulators on callus induction rate of anther and plant regeneration of Pansy were investigated.The results showed that 20 min with 0.1% HgCl2 was an ideal bud surface sterilization time,and obvious difference was detected between two genotypes in their effect on callus initiation of anther,but the overall rate of callus induction was not high.It may be suggested that the kind and concentration of hormones and culture conditions should be key factors for successful anther culture of pansy.