烟草NtLS基因RNA干扰表达载体的构建及遗传转化

Construction of RNAi Vector of NtLS Gene and its Transformation in Tobacco

腋芽的形成涉及多个调控基因,其中关键基因编码的蛋白属于植物特有的GRAS蛋白家族,它的突变或功能缺失将影响腋生分生组织的形成。笔者利用RACE技术克隆了烟草NtLS基因(GenBank登录号EU935581),NtLS与调控植物腋生分生组织形成的基因LS/LAS/MOC1序列高度相似。本研究从该基因上选取干扰片段,构建了含有反向重复序列的RNAi干扰表达载体,并通过农杆菌侵染的方法转染烟草W38,以期为NtLS基因的功能研究奠定基础。

Axillary bud formation involves many regulatory genes,one of key factors belongs to the plant specific protein family GRAS.Mutation or function missing of these genes would affect the axillary formation.The tobacco NtLS gene（EU935581） was cloned by RACE technology in our lab.The sequences of NtLS are highly similar to those of LS/LAS/MOC1,which regulate the formation of axillary meristems.The interference fragments were chosen from NtLS and the RNAi expression vectors were constructed and transformed into Nicotiana tabacum W38 by agrobacterium-mediated leaf disc transformation methods,to form basis for further study of NtLS function.