溃疡性结肠炎组织中核因子-kB和白细胞介素-13的表达及其意义

Expressions of Nuclear Factor kB and Interleukin - 13 in Colon Tissues of Ulcerative Colitis and Its Implication

目的研究核因子-kBp65（nuclear factor kB p65，NF—kBp65）、白细胞介素-13（IL-13）在溃疡性结肠炎（UC）结肠组织中的表达、分布，以及与UC病变程度的关系，探讨它们在UC发生发展中的作用。方法分别用免疫组化SP法和ELISA法检测活动期UC（46例）及正常对照（28例）组织中NF—kBp65和IL-13的表达情况。结果UC组结肠粘膜NF—kBp65的表达活性明显高于正常对照组，二组间有显著性差异（P〈0．05），而IL-13表达水平明显低于正常对照组（P〈0．01）。UC病情程度越重，NF—KBp65表达的阳性率越高，而IL-13表达越低，NF—kBp65和IL-13表达具有明显的负相关性（r=-0．768，P〈0．01）。结论NF—kBp65的表达与IL-13呈负相关，均参与了UC的发生和发展。NF—kBp65、IL—13反映了疾病的炎症程度，并且可作为病情严重程度评定的指标。IL-13的表达可能由NF—kBp65调控。

Objective To determine the levels of nuclear factor kB 1365 （ NF - kB p65 ） and interleukin - 13 （IL- 13）in colon tissue of patients with ulcerative colitis（UC） and to explore the relationship among the expressions of NF - kB p65 and IL - 13 and the severity of disease. Methods The levels of NF - kB p65 and IL - 13 in colon mucosa of UC patients were detected by immunohistochemistry method and ELISA respectively. Results The levels of NF - kB p65 in colon mucosa of UC patients were much higher than that of normal controls. The expressions of NF - kB p65 of UC patients were higher in involved mucosa than in corresponding normal mucosa. The levels of IL - 13 in colon mucosa of UC patients were much lower than that of normal controls. The expressions of IL - 13of UC patients were lower in involved mucosa than in corresponding normal mucosa. The expressions of NF - kB 1365 and IL - 13 were related to the extent of lesion and the degree of disease. The expression of NF - kB p65 was significantly nega- tive correlated with that of IL - 13 （ r = - 0. 768,P 〈 0. O1 ）. Conclusions The induction of NF - kB p65 and IL - 13 was involved in process of inflammation and development of UC. Regulating mechanisms of IL - 13 expression may be related with the induction of NF - kB 1o65.