摘要
Hg2+可猝灭1,8-蒽二磺酰胺的荧光,故加入GSH后,GSH所带的巯基(—SH)和Hg2+发生络合,夺取了与1,8-蒽二磺酰胺络合的Hg2+,使体系的荧光强度增大,据此可以快速地测定GSH的含量。在pH6.5的Na2HPO4-NaH2PO4缓冲溶液中,1,8-蒽二磺酰胺-Hg2+体系荧光强度的增强值与GSH的浓度在5.0×10-5～5.0×10-4 mol.L-1范围内呈线性关系,检测限为4.16×10-6 mol.L-1。
The reaction of glutathione（GSH） with Hg2＋ and the effect of GSH on the fluorescent systems,i.e.1,8-anthracene disulfonamide-Hg2＋ were studied.Hg2＋ can quench the fluorescence of 1,8-anthracene disulfonamide.With addition of GSH to which the mercapto（—SH） has a firmer combination with Hg2＋,GSH would wrested the Hg2＋ by combining with 1,8-anthracene disulfonamide,resulting in the recovery of fluorescence.With this method,GSH was detected rapidly.In the phosphate buffer（pH6.5） solution,a good linear relationship was obtained from 5.0×10-5 to 5.0×10-4 mol·L-1 for GSH,and the detection limit was 4.16×10-6 mol·L-1.
出处
《青岛科技大学学报（自然科学版）》
CAS
2011年第4期372-374,378,共4页
Journal of Qingdao University of Science and Technology:Natural Science Edition
基金
国家自然科学基金项目(21075073)
山东省自然科学基金重点项目(ZR2010BZ006)
关键词
谷胱甘肽
汞离子
1
8-蒽二磺酰胺
荧光光谱
glutathione
mercury ions
fluorescence intensity
1
8-anthracene disulfonamide
