基于SDS增溶的共聚物胶束中辅酶Q_(10)含量分析

Determination of Content of Coenzyme Q10 in Copolymer Micellers with SDS Solubilization

对酪蛋白-葡聚糖共聚物胶束作为输送体系而言,所包埋营养素的总量是评价其质量的重要指标。表面活性剂十二烷基硫酸钠(SDS)在将共聚物胶束解体的同时使辅酶Q10增溶于水中,从而便于进行定量分析。实验结果表明:当SDS浓度为0.31 mol/L、孵育温度30℃、硼氢化钠7mg/mL、用量100μL、还原时间3~5 min时,SDS增溶法的加样回收率在(98.77±1.60)%^(95.90±0.80)%之间。同时,共聚物中酪蛋白单元的内源荧光和外源荧光光谱分析显示,当SDS浓度为0~0.5 mol/L时,SDS会部分改变酪蛋白单元的疏水结构;且SDS浓度为0.31 mol/L时,SDS与酪蛋白单元疏水区的相互作用几乎不受温度的影响。

The total content of nutrient is an important index for evaluating the quality of casein- dextran copolymer micellers as a delivery system. Sodium dodecyl sulfate （SDS） as a surfactant can be used to disorganize copolymer micellers and make coenzyme Q10 solubilize in water for quantitative analysis. In this study, the recovery rate of coenzyme Q10from blank casein-dextran copolymer micellers ranged from （98. 77± 1.60） % to （95.90±0.80） % with conditions as follows： SDS concentration 0. 31 mol/L, incubation temperature 30 ℃, sodium borohydride （7 mg/mL） dL, reduction time 3-5 rain. The analysis of synchronous and exogenous fluorescence spectra indicated that the hydrophobic region of casein unit of copolymer was partly changed during solubilization at SDS concentration form 0 to 0. 5 mol/L. The interaction between SDS and hydrophobic region was not influenced by temperature when the concentration of SDS was 0. 31 mol/L.