大孔树脂分离纯化发酵液中虫草素的研究

Studies on Purification of Cordycepin from Fermented Broth by Macroporous Resin

采用大孔树脂富集纯化北冬虫夏草发酵液中的虫草素,通过比较发现6种大孔树脂中NKA-Ⅱ型大孔树脂对虫草素的吸附与解吸效果最好。静态和动态参数优化结果表明,NKA-Ⅱ型树脂纯化虫草素的最佳吸附平衡时间为6 h,解吸平衡时间为3 h。优化后的动态参数为:以1 BV/h流速上样吸附,体积分数10%乙醇除杂,70%乙醇以4 BV/h的流量洗脱。该工艺所得样品虫草素质量分数达35%,纯度提高了10倍,虫草素回收率达90%以上,经反复结晶后得到纯度大于98%的虫草素。

In this study,macroporous resin was used to extract cordycepin from fermentation broth of Cordyceps militaris.By compared with the adsorption and desorption effects of six kinds of macroporous resins to cordycepin,NKA-Ⅱ macroporous resin exhibited the excellent characteristics for cordycepin purification.Through optimization of static and dynamic parameters,the time of adsorption and desorption equilibrium of cordcepin by the NKA-II resin was 6h and 3h,respectively.The current velocity of adsorption on the column was 1 BV/h,and the eluting reagent was 70% ethanol with 4BV/h eluting rate after eliminating the impurity by 10% ethanol.By combination of those optimum conditions,the content of cordycepin,and the recovery rate achieved at 35% and 90%,respectively.Furthermore,the purity of Cordycepin was 98% through recrystallization for several times.