口腔鳞状细胞癌患者60例血清MMP-9和TIMP-1水平及其临床意义

The Level of Serum MMP-9 and TIMP-1 in 60 Cases with Oral Squamous Cell Carcinoma and its Clinical Significance

[目的]探讨基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶组织抑制因子-1(TIMP-1)在口腔鳞状细胞癌(OSCC)中的水平及意义。[方法]用ELISA法检测60例OSCC患者、26例口腔非典型增生患者术前及30名健康对照者血清中MMP-9、TIMP-1水平。[结果]OSCC患者血清MMP-9水平为21.67±4.21ng/ml,显著性高于口腔非典型增生组(15.43±2.35ng/ml)和正常对照组(11.29±4.78ng/ml)(P<0.05)。三组间TIMP-1水平及MMP-9/TIMP-1值无统计学差异。OSCC患者淋巴结转移组MMP-9水平为24.71±4.08ng/ml,显著高于未转移组的17.68±3.86ng/ml(t=6.77,P<0.01);肿瘤直径>3cm者MMP-9水平高于肿瘤直径≤3cm者(24.13±4.13ng/mlvs18.85±4.31ng/ml,t=4.84,P<0.01)。[结论]OSCC患者血清MMP-9水平显著升高,检测OSCC患者血清MMP-9水平有助于判断OSCC发生、转移。

[Purpose] To investigate the level of matrix metalloproteinase 9（MMP-9） and tissue inhibitor of metalloproteinase 1（TIMP-1） in oral squamous cell carcinoma（OSCC） and its significance. [Methods] The level of serum MMP-9 and TIMP-1 in 60 cases with OSCC preoperation, 26 cases with atypical hyperplasia of oral preoperation, and 30 healthy control was examined by ELISA. [Results] The level of serum MMP-9 in OSCC （21.67±4.21 ng/ml） was significantly higher than that in atypical hyperplasia of oral （15.43±2.35ng/ml） and healthy controls （11.29±4.78ng/ml） （P0.05）. There wasn’t significantly difference in TIMP-1 level and MMP-9/ TIMP-1 value among the 3 groups. The level of serum MMP-9 in patients with lymph node metastasis was higher than that in patients without lymph node metastasis with significant difference （24.71±4.08ng/ml vs 17.68±3.86ng/ml; t=6.77,P〈0.01）, and serum MMP-9 in patients with tumor diameter3cm was higher than that in patients with tumor diameter≤3cm （24.13±4.13 ng/ml vs 18.85±4.31 ng/ml; t=4.84,P〈0.01）. [Conclusion] The level of serum MMP-9 is significantly increased in cases with OSCC. Detection serum MMP-9 might be helpful to predict occurrence and metastasis of OSCC.