新城疫病毒作用下卵巢癌裸鼠移植瘤Survivin、Caspase-3的表达

The Expression of Survivin and Caspase-3 in the Ovarian Cancer Nude Mice Transplanted Tumor by Newcastle Disease Virus

目的研究新城疫病毒(Newcastle disease virus,NDV)对卵巢癌裸鼠移植瘤Survivin、Caspase-3表达的影响。方法①细胞培养:人卵巢癌细胞SKOV-3细胞的培养;②建立裸鼠移植瘤模型;③实验分组:分为实验组与对照组,实验组为肿瘤内注射NDV病毒抗卵巢癌;④应用免疫组化SP法观察Survivin、Caspase-3的表达。结果 Survivin表达实验组与对照组均为阳性,实验组明显弱于对照组,差异有显著性(P<0.05);Caspase-3表达实验组均为阳性,对照组1例表达,实验组明显强于对照组,差异有显著性(P<0.05)。结论 NDV可诱导肿瘤细胞凋亡,抑制其生长;其机制可能通过激活Caspase-3凋亡,抑制Survivin抗调亡作用。

Objective To investigate the expression of Survivin and Caspase-3 in the ovarian cancer nude mice injected by Newcastle disease virus. Methods SKOV-3 tumor cells were cultured; the model of SKOV-3 transplanted tumor model in the nude mice were established. We injected the NDV into the transplanted tumor in experiment group, water was injected in control group; Immunohistochemistry SP was used to detect the expression of Survivin and Caspase-3. Results The expression of Survivin was positive in both A and B group and the stain result in group A was lighter than in group B （ P 〈 0.05 ）. The expressions of Caspase-3 were all positive in experiment group, only had one in control group and experiment group was inferior than control group （ P 〈 0.05 ）. Conelusion When NDV is injected into, apoptosis is induced and the growth of neoplastic cell is inhibited. The mechanisms may be through activation of Caspase-3 and inhibition of anti-apoptosis effect of Survivin.