四川凉山静脉吸毒人群HIV感染者中HLA-B*5701阳性率的研究

Prevalence of human leukocyte antigen B site 5701 allele(HLA-B*5701)in HIV infected intravenous drug users(IDUs) in Liangshan of Sichuan province

目的了解四川凉山彝族自治州静脉吸毒人群(IDUs)艾滋病病毒(HIV)感染者中,人白细胞抗原I类基因B座位5701等位基因(HLA-B*5701)的阳性率,并与国内外其他民族的研究数据进行比较。方法采用序列特异性引物(SSP)对基因组DNA进行聚合酶链式(PCR)扩增,通过琼脂糖凝胶电泳读取结果,电泳条带呈HLA-B*5701阳性的再通过测序进行HLA-B分型验证。结果 1 043名HIV阳性感染者中,5人携带HLA-B*5701等位基因,阳性率为0.479%。其中汉族人口230人,3例携带HLA-B*5701等位基因(阳性率1.30%);彝族人口813人,2例携带HLA-B*5701等位基因(阳性率0.246%)。结论凉山IDUs人群HIV感染者中HLA-B*5701的阳性率较低,低于国外对黑种人及白种人的研究数据,汉族人群与彝族人群相比较差异无统计学意义。

Objective To study the prevalence of HLA-B＊ 5701 in HIV infected intravenous drug users （IDUs） in Liangsha of Sichuan province in comparison with data of domestic and foreign researchers. Methods HLA-B＊ 5701 screening was performed using multiplexed sequence specific primers PCR（SSP-PCR） method. The results were read by agarose gel electrophoresis, with additional DNA sequencing for IDUs whose agarose gel electrophoresis findings were positive. Results Of the 1 043 HIV positive IDUs 5 carried HLA-B＊ 5701 allele, with a prevalence rate of 0. 479 %. Of the 230 IDUs of Han ethnic , 3 carried HLA-B＊ 5701 allele （1.30%） ; of the 813 IDUs of Yi ethnic 2 carried HLA-B＊ 5701 allele （0. 246%）. Conclusion The prevalence of HLAB＊ 5701 in HIV infected IDUs in Liangshan was lower than that in the black and white people as identified by foreign researchers, and there was no significant difference between Han and Yi populations in this study.