摘要
目的:探讨在1%氧浓度下及6%氧浓度下体外培养正常妊娠孕妇胎盘绒毛组织培养液中代谢足迹的变化。方法:选择正常妊娠孕妇27例,在胎盘边缘、中间、近脐带处取绒毛组织,分别在1%氧浓度下及6%氧浓度下培养96 h,采用高效液相色谱-质谱法检测培养液中代谢产物,观察不同氧浓度下培养的绒毛组织代谢足迹的变化。结果:1%氧浓度和6%氧浓度下分别取自同一胎盘边缘、中间、近脐带三点处胎盘绒毛组织代谢足迹比较,无统计学差异(P>0.05);1%氧浓度与6%氧浓度下培养的孕妇胎盘绒毛组织代谢产物比较,发现545个代谢物有统计学差异(P<0.05)。结论:代谢组学提供了从另一个角度研究胎盘功能的方法,应用严谨的实验技术和适当的统计学分析,能确定体外培养的胎盘组织在不同氧浓度下的代谢差异,从而研究与胎盘病理学相关的改变。
Objective： To explore the changes of metabolic footprint of placental villous tissues （from normal pregnant women） culture in vitro in culture solution under the circumstance of 1% oxygen concentration and 6% oxygen concentration. Methods： 27 normal pregnant women were selected, their villous tissues were obtained from the margin and middle part of placenta and the the placental part around umbilical cord, then the villous tissues were cultured under the circumstance of 1% oxygen concentration and 6% oxygen concentration for 96 hours, high performance liquid chromatography/mass spectrometry was used to detect the metabolic products in culture solution, the changes of metabolic footprint of placental villous tissues culture in vitro under the circumstance of different oxygen concentrations were observed. Results： There was no significant difference in the metabolic footprint of placental villous tissues obtained from the same part of placenta between 1% oxygen concentration group and 6% oxygen concentration group （P 〉 0. 05 ） . There was significant difference in 545 metabolic products of placental villous tissues between 1% oxygen concentration group and 6% oxygen concentration group （P 〈 0. 05） . Conclusion： Metabonomics provides a method to research placental function from another perspective, precise experimental techniques and proper statistical analysis can confirm the metabolic difference of placental tissues culture in vitro under the circumstance of different oxygen concentrations and research the changes related to placental pathology.
出处
《中国妇幼保健》
CAS
北大核心
2011年第25期3949-3952,共4页
Maternal and Child Health Care of China
基金
深圳市科技计划项目〔201003113〕
