HPLC法测定盐酸普萘洛尔片的含量

Determination of Propranolol Hydrochloride Tablets by HPLC

目的建立测定盐酸普萘洛尔片含量的高效液相色谱法。方法采用SHIMADZU Shim-pack VP-ODS C18色谱柱(250mm×4.6mm,5μm),以甲醇-含0.1%庚烷磺酸钠的0.05mol/L磷酸二氢钾溶液(57∶43)为流动相,流速为1.0mL·min-1,检测波长为290nm。结果盐酸普萘洛尔在50.17～501.7μg/ml范围内与峰面积呈良好的线性关系,r=0.9999(n=6),平均加样回收率为99.43%,RSD为1.2%(n=9)。结论该方法简便、准确,专属性强,重现性好,可用于盐酸普萘洛尔片含量测定。

Objective To establish the determination method of Propranolol Hydrochloride Tablets by HPLC.Method The quantitative determination was performed on a SHIMADZU Shim-pack VP-ODS C18 column（250mm×4.6mm,5μm）.using methanol：0.05mol/L potassium dihydrogen phosphate containing 0.1% sodium heptanesulfonate solution as mobile phase （57∶33） with a flow rate of 1.0mL·min^-1.And the UV detective wavelength was set 290nm.Result The method showed a good linear relationship within the range of 50.17ug/ml～501.7ug/ml, r=0.9999（n=6）.The average recovery was 99.43%, RSD =1.2% （n=9）.Conclusion The method is simple, accurate, reliable, reproducible and selective and could be used for quantitative determination of Propranolol Hydrochloride Tablets.