汉防己甲素对瘢痕成纤维细胞有丝分裂的调控

Inhibition effect of tetrandrine on division of fibroblast from keloid

目的:探讨汉防己甲素对瘢痕成纤维细胞有丝分裂的影响。方法:将不同浓度梯度的汉防己甲素与瘢痕成纤维细胞体外共培养,不同时间点分别取细胞标本检测其分裂指数,并使用流式细胞仪检测其细胞周期及不同周期细胞分布。将以上检测值与空白对照及阳性对照比较。结果:汉防己甲素组在各个时间点的分裂指数均较空白对照组明显下降,使峰值时间缩短,具有作用浓度及时间依赖性,汉防己甲素组G0-G1期细胞比例降低,S期及G2-M期细胞比例明显增多,S期与G2-M期时相时间明显延长。结论:汉防己甲素使成纤维细胞分裂停滞于G2-M期,抑制细胞分裂,且具有明显浓度及时间依赖性。

Objective：To probe the effect of tetrandrine on mitosis of scar fibroblast.Methods：Fibroblast was cultured with various concentrations of tetrandrine,then the cultured fibroblast samples were gathered on different days of time gradient.Mitosis index was determined by light microscopy.Cell cycle and its distributions were analyzed by flow cytometer.Results：Mitosis index of tetrandrine group was reduced obviously compared with blank group at all time points and the peak time was shortened in a dose-and time-dependent manner.In the flow cytometry analysis,it was found that tetrandrine could promote fibroblast to enter S and G2-M phase from G0-G1 phase and extend the time of S G2-M phase distinctly.Conclusion：Tetrandrine can block the division of fibroblast at G2-M phase in a dose-and time-dependent manner.