L-谷氨酰胺与肝脏大部分切除后的再生

Effects of orally supplied L-glutamine on hepatic regeneration in partial hepatectomic rats

背景:L-谷氨酰胺作为DNA和谷胱甘肽等合成的氮前体,在肝组织再生,肝细胞增殖的过程中扮演着极其重要的角色。目的:观察经饮食由来补充L-谷氨酰胺对大鼠肝脏大部切除后肝再生能力的影响。方法:Wistar大鼠随机分组3组,L-谷氨酰胺组和L-丙氨酸组大鼠肝切除前分别灌服10%L-谷氨酰胺或10%L-丙氨酸,肝切除后继续加入饮用水中饮用,对照组肝切除前后均使用饮用水。结果与结论:大鼠肝切除后72hL-谷氨酰胺组肝再生率明显高于对照组及L-丙氨酸组(P<0.05)。肝切除后24h和72hL-谷氨酰胺组肝细胞增殖均明显高于对照组和L-丙氨酸组(P<0.01;P<0.05)。肝切除后24h和72h总RNA水平在两种氨基酸与对照组之间差异无显著性意义。肝切除后72h基因组DNA的含量L-谷氨酰胺组显著高于对照组和L-丙氨酸组(P<0.05)。提示肝损伤围手术期投用高浓度L-谷氨酰胺对大鼠肝再生有促进作用,而投用L-丙氨酸则没有此作用。

BACKGROUND：As a necessary nitrogen provider in the processes of DNA and glutathione （GSH） biosynthesis, also a nitrogen source of plasma ammonia formation, L-glutamine is considered to play important roles in hepatic cell proliferation. But the functions and the mechanisms of L-glutamine in liver regeneration are not clearly studied and still need further investigation. OBJECTIVE：To investigate the effects of dietary supplied L-glutamine on hepatic regeneration in a rat model of partial hepatectomy. METHODS：Wistar rats were randomly divided into three groups. Before partial hepatectomy, rats in the L-glutamine and L-alanine groups were orally administered 10% L-glutamine and 10% L-alanine, respectively. After partial hepatectomy, rats from these two groups were given water containing 10% L-glutamine and 10% L-alanine, respectively. The control group rats were allowed to drink water before and after partial hepatectomy. RESULTS AND CONCLUSION：At 72 hours after partial hepatectomy, restituted liver mass was significantly greater in the L-glutamine group than in the control and L-alanine groups （P 0.05）. At 24 and 72 hours after partial hepatectomy, hepatocyte proliferation was more obvious in the L-glutamine group than in the control and L-alanine groups （P 0.01;P 0.05）. At 24 and 72 hours after partial hepatectomy, there was no significant difference in total RNA level between L-glutamine and L-alanine groups and control groups. At 72 hours after partial hepatectomy, genomic DNA content was significantly greater in the L-glutamine group than in the control and L-alanine groups （P 0.05）.These results showed that application of high concentration L-glutamine in the perioperative stage of liver injury promotes hepatic regeneration in rats after partial hepatectomy, but L-alanine does not produce this effect.