BALB/c裸鼠与SCID小鼠皮下种植人肝癌模型的比较

Subcutaneous implantation of human liver neoplasms in BALB/c naked mice and SCID mice

背景:建立良好的肝癌模型是进一步研究肝癌诊断、治疗的基础,目前各种模型较多,但是进行评价的很少。目的:比较两种免疫缺陷小鼠种植性肝癌的成瘤情况。方法:免疫磁珠法从Huh7肝癌细胞中分选出CD133+肝癌干细胞,取1×106个细胞分别种植于BALB/c祼鼠及SCID小鼠颈背部皮下,观察两组20d的成瘤及死亡情况,游标卡尺分别测量种植性肝癌的直径,以大于0.5cm为成瘤成功。结果与结论:24只BALB/c裸鼠中18只成瘤成功;24只SCID小鼠中16只成瘤成功。行χ2检验,两组成瘤效果差异无显著性意义(P>0.05),两组死亡情况比较,差异有显著性意义(P<0.05)。结果提示BALB/c组死亡率低,且价格低廉,更适合建立肝癌皮下种植模型。

BACKGROUND：A good model of liver neoplasms is the basic for further diagnosis and treatment of liver carcinoma. There have been many types of models, but few of them are evaluated. OBJECTIVE：To compare the tumor formation in two kinds of immune deficiency mice in which human liver neoplasms were implanted. METHODS：CD133＋liver carcinoma stem cells were sorted from Huh7 hepatoma cells by immunomagnetic beads and 1×106 cells were implanted into the back skin of BALB/c naked mice and SCID mice. Within 20 days after implantation, the tumor formation and deaths of naked mice were investigated, and the maximal diameter of liver neoplasms was measured through the use of vernier caliper, and tumor with a diameter of 0.5 cm was considered successful. RESULTS AND CONCLUSION：Among 24 BALB/c naked mice, tumor was successfully induced in 18 mice, and among 24 SCID mice, tumor was successfully induced in 16 mice. Chi-square test showed that there was no significant difference in tumor formation （P 0.05）, but significant difference in deaths exist （P 0.05）, between these two types of mice. These results showed that BALB/c mice are superior to SCID mice in establishment of subcutaneous implantation of liver carcinoma because they show low mortality and cost less.