DCs在EAE鼠免疫耐受的症状及分泌细胞因子的变化

Effect of cytokines in immunotolerance induced by dendritic cells in EAE

目的研究树突状细胞(dendritic cells,DCs)在实验性变态反应性脑脊髓炎(experimental allergic encephalomyelitis,EAE)免疫耐受对细胞因子的影响。方法建立EAE组及耐受组模型,评价EAE模型及耐受鼠发病情况。采用ELISA双夹心法测量各组脾脏DCs培养液中细胞因子水平。结果 EAE模型组全部发病,平均12～14天达高峰。EAE组脾脏DCs培养上清液的TNF-αI、CAM-1较耐受组明显增高,差异有显著意义(P<0.01,P<0.01);而IL-10水平耐受组较EAE组显著增高,组间比较有统计学差异(P<0.05)。结论 DCs调控免疫反应并影响细胞因子分泌水平,可能在MS免疫耐受机制中起重要作用。

Objective To study the effect of cytokines in immunotolerance induced by dendritic cell in experimental allergic encephalomyelitis（EAE）. Methods Two groups including the model of EAE and immunotolerance were established and the clinical features were judged. The levels of cytokines （TNF-α, ICAM-land IL-10） produced by splenic DCs were detected by ELSIA. Results All rats of EAE group were developed a tipical course of EAE. The course of high peak was 12 - 14 days. The levels of TNF-α,TCAM-1 in EAE group was higher than those in group of immunotolerance. The differences were significant（P 〈0.01, P 〈0. 01 ）. The level of IL-10 in EAE group was lower than that in group of immtmotolerance. The differences were significant（P 〈 0. 05 ）. Conclusion DCs can probably affect the production of cytokines by controlling the type of immune responses. DCs may play an important role in immunotolerance of EAE.