复方斑蝥制剂对直肠癌微淋巴管生成影响的实验研究

Effect of Fufangbanmao on lymphangiogenesis in rectal cancer

目的通过建立未分化人直肠癌HR8348原位移植裸鼠模型,应用复方斑蝥制剂治疗,观察其对裸鼠原位移植人直肠癌微淋巴管生成的影响。方法建立人直肠癌HR8348原位种植BALB/C裸鼠模型80只,随机分为两组,每组40只,分别腹腔内注射0.9%氯化钠溶液、复方斑蝥制剂8 mg/kg,2次/周。8周后处死裸鼠,采用定量免疫组化染色检测肾小球足突细胞膜黏蛋白;计数微淋巴管密度(LM-VD)值。结果复方斑蝥制剂组肾小球足突细胞黏蛋白值较0.9%氯化钠溶液组显著下降(P<0.05)。复方斑蝥制剂组LMVD值为3.98±0.42,较0.9%氯化钠溶液组显著下降(7.52±1.25,P<0.05)。结论复方斑蝥制剂能抑制直肠癌微淋巴管生成,抑制肿瘤的生长和转移。

Objective To evaluate the inhibitory effect of Fofangbanmao on lymphangiogenesis in rectal cancer using orthotopic implantated tumor models of BALB/C nude mice. Methods A BALB/C nude mouse model of transplanted in situ human rectal cancer was established. Eighty nude mice were divided into two groups with 40 each： normal saline or 8 mg/kg of Fufangbanmao were injected in, repectively,twice a week for 8 weeks. At the end.of the 8th week, all mice were sacrificed for detection of lymphatic microvessel density （ LMVD）, and podoplanin was detected by immunohistoehemical staining. Results In comparison with control group, the concentrations of podoplanin in Fufangbanmao group decreased significantly than those in control group （P 〈 0. 05 ）. The number of LMVD in Fufangbanmao group was 3.98 ± 0. 42, which was significantly lower than that in control group（7.52 ± 1.25,P 〈 0.05）. Conclusion Fufangbanmao can inhibit the growth of rectal cancer by interfering lymphangiogenesis.