肉桂醛抗腺病毒作用研究

Anti-adenovirus effect of cinnamaldehyde

目的:研究肉桂醛抗腺病毒(ADV)作用。方法:采用噻唑蓝(MTT)比色法和病毒增殖抑制实验观测肉桂醛抗ADV作用;免疫荧光法检测肉桂醛对ADV六邻体(hexon)蛋白表达的影响。结果:(1)MTT和病毒增殖抑制实验结果表明肉桂醛在3种作用方式(除细胞保护作用方式)下能够增强宿主细胞存活率,降低病毒滴度,且细胞存活率与药物浓度呈正相关,肉桂醛对宿主细胞无保护作用,不能阻断病毒进入细胞。(2)肉桂醛(除细胞保护作用方式)组与病毒组比较hexon蛋白的表达明显降低(P<0.05)。结论:肉桂醛有抗ADV作用,但却对宿主细胞无保护作用,肉桂醛抗ADV作用可能与调节hexon蛋白的表达有关。

AIM： To investigate the anti - adenovirus effect of cinnamaldehyde in vitro. METHODS ： The anti - adenovirus effect of cinnamaldehyde was measured by 3 - （4,5 - dimethyhhiazol - 2 - yl） - 2,5 - diphenyhetrazolium bromide （MTT） method and virus proliferation inhibition experiment. The expression of hexon protein was measured by immunohistochemistry. RESULTS ： The results of MTT and virus proliferation inhibition experiment showed that cinnamaldehyde enhanced the survival rate of the host cells, and decreased adenovirus titer by the ways of direct virus inactivation, anti - adenovirus in the stage of biosynthesis and adsorption of the adenovirus. The viability of the host cells had a positive correlation with the drug concentration under the condition of virus infection. Cinnamaldehyde did not block the.virus uptake into host cells, and did not protect the host cells from infection. The expression of hexon protein was significantly lower （P 〈 0. 05 ） in cinnamaldehyde group than that in virus group. CONCLUSION： Cinnamaldehyde has anti - adenovirus effect, but can not directly protect host cells. The anti - adenovirus effect of cinnamaldehyde may be related with the inhibition of hexon protein expression.