白细胞分化抗原14基因多态性与严重胸部创伤后并发多器官功能障碍综合征的相关性

Association between the Polymorphisms of Cluster of Differentiation 14 Gene Promoters and the Susceptibility of Multiple Organ Dysfunction Syndrome after Severe Chest Trauma

目的探讨白细胞分化抗原14(CD14)基因启动子区-1145A/G、-159C/T多态性与重庆地区严重胸部创伤(SCT)患者并发多器官功能障碍综合征(MODS)的相关性。方法采用限制性片段长度多态性-聚合酶链式反应技术,检测106例SCT患者(其中47例并发MODS)CD14基因启动子区-159C/T、-1145A/G单核苷酸多态性位点基因型。结果 -1145G等位基因携带者并发MODS的可能性显著高于A等位基因携带者(P=0.033),G等位基因携带者MODS评分显著高于A等位基因携带者(显性遗传模式P=0.217,隐性遗传模式P=0.037)。-159T等位基因携带者MODS评分显著高于C等位基因携带者(显性遗传模式P=0.048,隐性遗传模式P=0.198)。-1145、-159等位基因型与MODS评分呈线性相关(P=0.043,P=0.046)。两个位点同时发生突变与只有1个位点发生突变的患者相比,MODS发生率差异有统计学意义(P<0.01),MODS评分差异无统计学意义(P=0.239)。结论 CD14基因启动子区-1145 A/G、-159C/T多态性与重庆地区汉族SCT患者并发MODS有相关性。

Objective To investigate the polymorphisms of cluster of differentiation 14（CD14） gene promoters and explore whether such polymorphisms are associated with the susceptibility to multiple organ dysfunction syndrome（MODS） in Chongqing population.Methods The single nucleotide polymorphisms of the promoter region of CD14 gene at position-1145 and-159 were detected using polymerase chain reaction-restriction fragment length polymorphism method in 106 patients with severe chest trauma,among whom 47 were with MODS.Results Trauma patients carrying G allele tended to have a higher risk of MODS than those carrying A allele at position-1145,the MODS scores in trauma patients carrying G allele were significantly higher than those carrying A allele（P=0.217 for dominant effect and P=0.037 for recessive effect）,and the MODS scores in trauma patients carrying T allele were significantly higher than those carrying C allele at position-159（P=0.048 for dominant effect and P=0.198 for recessive effect）.The genotypes of CD14 gene at positions-1145 and-159 were significantly correlated with the MODS scores（P=0.043,P=0.046）.Compare with single-point mutation,simultaneous two-point mutation had significantly higher risk of MODS（P0.01）,while the difference of MODS scores showed no statistical significance（P=0.239）.Conclusion The polymorhisms of CD14 gene promoters are associated with MODS after severe chest trauma in Chongqing population.