人脐带间充质干细胞的生物学特性和超微结构

Biological Features and Ultrastructure of Human Umbilical Cord Mesenchymal Stem Cells

目的分离和培养人脐带间充质干细胞(MSC),研究其生物学特性和超微结构。方法提取人脐带MSC,测定其增殖、周期和凋亡情况,采用透射电镜观察细胞的超微结构,酶联免疫吸附测定法检测细胞分泌血管内皮细胞生长因子(VEGF)、类胰岛素生长因子-1(IGF-1)、肝细胞生长因子(HGF)水平。结果人脐带MSC为成纤维细胞样,增殖能力强,超微结构分析其细胞代谢活跃,迁移分化能力强,能分泌VEGF、IGF-1和HGF等细胞因子。结论人脐带MSC能分泌多种抗凋亡因子,具有良好的生物学特性。

Objective To isolate and culture human umbilical cord mesenchymal stem cells（MSCs） and explore their biological features and ultrastructure.Methods After isolating MSCs from the human umbilical cord,the proliferation,cycle,and apoptosis were observed.The cell ultrastructure was observed under transmission electron microscope.The cytokines including vascular endothelial growth factor（VEGF）,hepatocyte growth factor（HGF）,and insulin-like growth factor-1（IGF-1） were detected using enzyme-linked immunosorbent assay.Results Human umbilical cord MSCs had fibroblast-like morphology and increased proliferation capability.Ultrastructural analysis showed that the MSCs had active cellular metabolism and strong migration and differentiation capabilities.Meanwhile,they could secrete anti-apoptotic cytokines such as VEGF,IGF-1,and HGF.Conclusion Human umbilical cord MSCs can secrete many anti-apoptotic cytokine and have good biological features.