膀胱癌受体酪氨酸激酶基因及蛋白的表达

Expressions of Receptor Tyrosine Kinases mRNA and Protein in Carcinoma of Bladder

目的检测膀胱癌受体酪氨酸激酶(RTKs)mRNA和蛋白的表达,探讨有前景的膀胱癌生物标志物及可能的药物靶点。方法分别采用实时定量PCR芯片技术及蛋白芯片技术,检测浸润性膀胱尿路上皮癌组织中RTKs mRNA及蛋白的表达,以正常膀胱组织作为对照。使用生物信息学方法对检测结果进行比较分析。结果 RTKs家族中的TG-FA、STAB1、SERPINE1、ANGPT2、SPINK5、ANGPTL1、PROK1、MDK、CXCL9、GRN、RUNX1、VEGFA、TGFB1在膀胱癌组织中的表达显著上调,EDIL3、PTN、CCL2、PDGFD、FGF13、KITLG、FGF2、SERPINF1、TNF显著下调。ALK、Btk、EphB2、ErbB4、PDGFR-α、ROS、Tie-2、Tyk2、VEGFR3蛋白在膀胱癌组织中高表达,FRK、Fyn、IGF-IR、InsulinR、Itk、JAK1、JAK3、LCK蛋白低表达。结论靶向血管内皮生长因子/血小板衍生生长因子药物可能在治疗膀胱癌方面发挥积极作用。

Objective To detect the expressions of receptor tyrosine kinases（RTKs） mRNA and protein and to explore potentially promising tumor markers and conceivable drug target in bladder cancer.Methods The expressions of RTKs mRNA and protein in tissue from invasive urothelial carcinoma of the bladder were examined by real-time quantitative PCR array and cytokine antibody array,with normal bladder tissue as control.The results were analyzed using bioinformatic approaches.Results The expressions of TGFA,STAB1,SERPINE1,ANGPT2,SPINK5,ANGPTL1,PROK1,MDK,CXCL9,GRN,RUNX1,VEGFA,and TGFB1 were obviously upregulated in bladder cancer tissue,while those of EDIL3,PTN,CCL2,PDGFD,FGF13,KITLG,FGF2,SERPINF1,and TNF were downregulated.ALK,Btk,EphB2,ErbB4,PDGFR-α,ROS,Tie-2,Tyk2,and VEGFR3 were over-expressed in bladder cancer,while FRK,Fyn,IGF-IR,Insulin R,Itk,JAK1,JAK3,and LCK were low-expressed.Conclusion Vascular endothelial growth factor/platelet-derived growth factor-targeted therapies may play an active role in treating carcinoma of bladder.