E2F3、MCM2及HIF-1α在结直肠癌中的表达及其相关性

Expression of E2F3, MCM2 and HIF-1α in colorectal carcinoma

目的:探讨转录因子E2F3、微型染色体维持蛋白(minichromosome maintenance proteins 2,MCM2)及缺氧诱导因子1α(hypoxia-inducible factor1α,HIF-1α)在结直肠癌、结直肠腺瘤、大肠正常黏膜组织中的表达及他们与结直肠癌临床病理因素之间的关系.方法:收集2007-01/2008-12佳木斯大学附属第一医院普通外科、肿瘤外科及消化内科肠镜室行手术切除并经病理证实的结直肠癌病理标本40例.应用免疫组织化学SP方法检测E2F3、MCM2及HIF-1α在结直肠癌(40例)、结直肠腺瘤(20例)及大肠正常黏膜组织(20例)中的表达.结果:E2F3的阳性表达率在结直肠腺瘤组织与结直肠癌组织中均显著高于正常组织(30.0%vs0.0%,57.5%vs0.0%,P<0.01).MCM2的阳性表达率在结直肠腺瘤组织与结直肠癌组织中均显著高于正常组织(45.0%vs5.0%,87.5%vs5.0%,P<0.01);HIF-1α的阳性表达率在结直肠腺瘤组织与结直肠癌组织中均高于正常组织(30.0%vs5.0%,67.5%vs5.0%,P<0.05).E2F3、MCM2、HIF-1α的表达水平与结直肠癌的浸润程度、淋巴结转移、Dukes分期、组织学分级相关(均P<0.05),与患者的年龄、性别及肿瘤的大小则无相关性.在结直肠癌组织中E2F3与MCM2的表达呈正相关(r=0.440,P<0.01),与HIF-1α的表达呈正相关(r=0.375,P<0.05);HIF-1α与MCM2的表达呈正相关(r=0.383,P<0.05).结论:E2F3、MCM2及HIF-1α在结直肠癌组织中均为高表达且呈正相关,提示在结直肠癌的发展过程中,三者可能通过各自不同功能作用于肿瘤细胞,还可能通过一种或多种途径协同促进肿瘤的发展演变.检测结直肠癌组织中三者的表达,可为结直肠癌早期诊断、评价预后提供理论依据,为防癌普查及基因靶向治疗提供一种新的途径.

AIM： To investigate the expression of E2F tran- scription factor 3 （E2F3）, minichromosome main- tenance protein 2 （MCM2） and hypoxia-inducible factor-1α （HIF-1α） and to analyze their correlation with clinical and pathophysiological param- eters in colorectal carcinoma and adenoma. METHODS： Imrnunohistochemistry was per- formed to detect the expression of E2F3, MCM2 and HIF-1α in specimens of colorectal carcinoma （n = 40）, colorectal adenoma （n = 20） and normal colorectal mucosa （n = 20）. RESULTS： The positive rates of E2F3 and HIF- 1α expression in colorectal carcinoma were sig-nificantly higher than in colorectal adenoma and normal colorectal tissue （E2F3： 30%, 57.5% vs 0.0%, both P 〈 0.01; MCM2： 45.0%, 87.5% vs 5.0%, both P 〈 0.01; HIF-1α： 30.0%, 67.5% vs 5.0%, both P 〈 0.05）. The expression of E2F3, MCM2 and HIF-1α in colorectal carcinoma was positively correlated with lymph node metastasis, depth of invasion and Dukes stage, but not with sex, age or tumor size （all P 〉 0.05）. There is a positive correlation between the expression of E2F3 and MCM2 （r = 0.440, P 〈 0.01）, between that of E2F3 and HIF-1α （r =0.375, P 〈 0.05）, and between that of HIF-1α and MCM2 （r = 0.383, P 〈 0.05） in colorectal carcinoma. CONCLUSION： The expression of E2F3, MCM2 and HIF-1α is high in colorectal carcinoma. There is a positive correlation among the expression of E2F3, MCM2 and HIF-1α in colorectal carcinoma.