期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

口腔鳞癌组织蛋白差异表达的蛋白组学测定 被引量：9

Proteomic determination of differentially expressed protain in oral squamous cell carcinoma
下载PDF
导出
摘要 目的应用蛋白质组学(proteomics)方法建立人口腔鳞癌组织与正常口腔黏膜组织中的差异蛋白质表达。方法收集10例口腔鳞癌组织及正常黏膜组织,提取蛋白质,进行双向凝胶电泳,经图像分析软件分析识别差异表达,应用液相色谱-质谱分析鉴定差异蛋白质。结果分析鉴定出S100A7、S100A8、S100A9在口腔鳞癌中高表达。结论 S100A7、S1000A8、S100A9在口腔鳞癌发生中发生了改变,为进一步筛选口腔鳞癌特异性的分子标志物打下了坚实的基础。 Objective To establish the two-dimensional electrophoresis profiles from human oral squamous cell carcinoma tissue and paired tumor adjacent normal oral mucosa tissue,and to identify differential expression of tumor associated proteins by proteomic analysis.Methods Gather 10 cases of human oral squamous carcinoma and normal oral mucosa tissue for proteome analysis,and carry out two-dimensional electrophoresis.The differentially expressed proteins were analyzed by liquid chromatography-mass spectrometry.Results 3 protein spots,S100A7、S100A8、S100A9 expressed more in oral squamous cell carcinoma.Conclusions S100A7、S100A8、S100A9 changed in the development of OSCC.Differential expression of proteins might be candidate biomarkers for diagnosis of oral squamous cell carcinoma,and proteomic technique was valuable for screening the diagnostic biomarkers.These data could be used to screen special biomarkers of OSCC.
作者 邱乐宏 马洪
机构地区 海南省海口市人民医院口腔中心 贵州省贵阳医学院口腔医学系
出处 《口腔医学》 CAS 2011年第8期453-455,共3页 Stomatology
基金 贵州省优秀科技教育省长专项资金资助[黔专合2006(116号)]
关键词 口腔鳞癌 双向凝胶电泳 蛋白质组 oral squamous cell carcinoma（OSCC） two-dimensional gel electrophoresis proeome
分类号 R739.81 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1邱蔚六.口腔颌面外科学[M].4版.北京:人民卫生出版社,2000:219-225.
  • 2Kuo MYP, Jeng JK, Chiang CP,et al. Mutations of Ki-ras oncogene codon 12 in betel quid chewing-related human squamous cell carcinoma in Taiwan [ J ]. J Oral Pathol Med, 1994,23 (2) :70 - 74.
  • 3Caulin C, Nguyen T, Longley MA, et al. Inducible activation of oncogenic K-ras results in tumour formation in theoral cavity [ J ]. Cancer Res ,2004,64 ( 15 ) :5054 - 5058.
  • 4Chambers G, Lawrie L, Cash P, et al. Proteomics: a new approach to the study of disease[ J]. J Patho1,2000,192 (3) :280 -288.
  • 5Mullner S, Nemann T, Lottspeich F. Proteomics -a new way for drug tar get discovery [ J ] . Arzneimitt-elforchung, 1998,48 ( 1 ) : 93 - 95.
  • 6Wu F, Chakravarti S. Diferential expression of inflammatory and fibrogenic genes and their regulation by NF-kappa inhibition in a mouse model of chronic colitis [ J ]. Immunol, 2007,179 ( 10 ) : 6988 - 7000.
  • 7Yui S,Nakatani Y, MikamiM. Calprotectin ( S100A8 /S100A9) , an inflamm a tory protein complex from neutrophils with a broad apop to sis-inducing activity [ J ]. Biol Pharm Bull ,2003,26 (6) : 753 - 760.
  • 8Gebhardt C, Breitenbach U, Tuckermann JP, et al. Calgranulins S100A8 and S100A9 are negatively regulated by glucocorticoids in ac-Fos-dependent manner and overexp ressed throughout skin carcinogenesis [ J ]. Oncogene ,2002,21:4266 - 4276.
  • 9Ehlermann P, Eggers K, Bierhaus A,et al. Increased proint-lammatory endothelial response to S100AS/A9 after preactivation through advanced glycation end products [ J ]. Cardiovasc Diabetol, 2006, 5,6.
  • 10Jinquan T, Vorum H, Larsen CG,et al. Psoriasin:a novel chemotactic protein [ J ]. J InVest Dermatol, 1996,107 ( 1 ) :5 - 10.

二级参考文献12

  • 1Chen GA,Gharib TG,Wang H,et al.Protein profiles associated with survival in lung adenocarcinoma.Proc Natl Acad Sci USA,2003,100(23):13537-13542.
  • 2Jiang D,Ying W,Lu Y,et al.Identification of metastasis-associated proteins by proteomic analysis and functional exploration of interieukin-18 in metastasis.Proteomics,2003,3(5):724-737.
  • 3Li C,Chen Z,Xiao Z,et al.Comparative proteomics analysis of human lung squamous carcinoma.Biochem Biophys Res Commun,2003,309(1):253-260.
  • 4Zhang H,Wang YP,Chen Y,et al.Identification and validation of S100A7 associated with lung squamous cell carcinoma metastasis to brain.Lung Cancer,2007,57(1):37-45.
  • 5Alowami S,Qing G,Emberley E,et al.Psoriasin (S100A7) expression is altered during skin tumorigenesis.BMC Dermatol,2003,3:1.
  • 6Celis JE,Rasmussen HH,Vorum H,et al.Bladder squamous cell carcinomas express psoriasin and externalize it to the urine.J Urol,1996,155(6):2113-2119.
  • 7Al-Haddad S,Zhang Z,Leygue E,et al.Psoriasin (S100A7) expression and invasion breast cancer.Am J Pathol,1999,155(6):2057-2066.
  • 8Enerback C,Porter DA,Polyak K,et al.Psoriasin expression in mammary epithelial cells in vitro and in vivo.Cancer Res,2002,62(1):43-47.
  • 9Luo CL,Xian XY,Liu DH,et al.CABYR is a novel cancer-testis antigen in lung cancer.Clio Cancer Res,2007,13(4):1288-1297.
  • 10Hu Ruide,Wu R,Deng J,et al.A small proline-rieh protein,spr1:specific marker for squamous lung carcinoma.Lung Cancer,1998,20(1):25-30.

共引文献5

同被引文献55

  • 1吴健民.对肿瘤标志物的再认识[J].中华检验医学杂志,2005,28(1):11-13. 被引量：116
  • 2常俊丽,杨广笑,何光源.蛋白质组学分离检测技术研究进展[J].武汉植物学研究,2006,24(3):261-266. 被引量：6
  • 3李兴,刘英,周贤卫,贺小燕,潘卫.糖尿病大鼠肾脏蛋白表达的双向电泳分析[J].贵阳医学院学报,2007,32(1):42-44. 被引量：1
  • 4颜子颖,王海林,译.精编分子生物学实验指南[M].北京:北京科学出版社,1998.305-307.
  • 5Klaas J, van Wijk. Challenges and prospects of plant proteomics [ J ]. Plant Physio1,2001,126 (2) :501 - 508.
  • 6Patel V, Leeththanakul C, Gutkind JS. New approaches to the un- derstanding of molecular basis of oral cancer[J].Crit Rev Oral Bi- ol Med,2001,12( 1 ) :55 -63.
  • 7Vokes EE,Weichselbaum RR,Lippman SM,et al. Head and neck cancer[J}. New Eng J Med,1993,328(3) :184 -194.
  • 8Hu S,Yu T,Xie Y,et al. Discovery of oral fluid biomarkers for hu- man oral cancer by mass spectrometry [ J ]. Cancer Genomics Pro- teomics ,2007,4 ( 2 ) :55 - 64 .
  • 9Hu S, Arellano M, Boontheung P,et al. Salivary proteomics for oral cancer biomarker discovery [ J ]. Clinic Cancer Res, 2008, 14 (19) :6246 -6252.
  • 10Brinkmann O, Kastratovic DA, Dimitrijevic MV, et al. Oral squa- mous cell carcinoma detection by salivary biomarkers in a Serbian population[J].Oral Oncol,2011,47( 1 ) :51 -55.

引证文献9

二级引证文献15

口腔医学
2011年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部