肺癌细胞株E-cadherin的表达状态及其与启动子甲基化的关系

Protein expression and promoter methylation status of E-cadherin in lung cancer cell lines

[目的]研究肺癌细胞株上皮钙粘蛋白(E-cadherin)的表达与基因启动子甲基化状态的关系。[方法]采用免疫细胞化学法检测肺癌细胞株NCI-H460、SPCA和A549的E-cadherin蛋白表达;半巢式甲基化特异性PCR法(MSP)和微流控芯片技术检测E-cadherin基因启动子甲基化状态。[结果]E-cadherin蛋白在NCI-H460、SPCA和A549肺癌株均呈弱表达,以A549细胞株表达最低;MSP法和微流控芯片技术在三种细胞株中均检测到E-cadherin基因启动子的甲基化,以A549细胞株启动子甲基化程度最高,二者结果相符。[结论]E-cadherin基因启动子甲基化可能是肺癌细胞株NCI-H460、SPCA和A549出现E-cadherin蛋白表达下调的原因。

[Objective] To investigate the relationship between the promoter methylation status and protein expression of E-cadherin in lung cancer cell lines.[Methods] The protein expressions of E-cadherin in lung cancer cell lines NCI-H460,SPCA and A549 were examined by immunocytochemistry.The promoter methylation status of E-cadherin was detected by methylation-specific PCR（MSP） and microfluidics.[Results] The weak expressions of E-cadherin in NCI-H460,SPCA and A549 were detected,the weakest in A549.The promoter methylation of E-cadherin occurred in all three cell lines and the highest percentage of methylation in A549,which was in agreement with the protein expression.[Conclusions] The promoter methylation can be a cause of E-cadherin its down-regulation in lung cancer cell lines.