正常关节软骨和骨关节炎关节软骨细胞中血管活性肠肽表达的比较

Comparison of vasoactive intestinal polypeptide expression between normal and osteoarthritic cartilages

背景:神经肽的发现给骨关节炎的治疗带来了新的希望,但神经肽的表达与骨关节炎发病以及软骨退变程度的关系尚不清楚。目的:观察血管活性肠肽在正常关节软骨和不同退变程度骨关节炎软骨中的表达,以及血管活性肠肽表达与骨关节炎发病及软骨退变程度的关系。方法:选取2007-03/11中南大学湘雅医院骨科进行关节置换的骨关节炎患者的关节软骨标本26个,选取因外伤行截肢的膝关节软骨或股骨颈暴力骨折的股骨头正常关节软骨标本10个为对照,根据大体观察凿取正常和骨关节炎不同退变程度软骨块50个,再根据关节软骨改良Mankin病理评分法进行分组,采用免疫组织化学染色检测软骨组织中血管活性肠肽的表达和分布。结果与结论:各关节软骨中均可见到血管活性肠肽阳性神经纤维,正常关节软骨中血管活性肠肽的表达明显高于骨关节炎关节软骨(P<0.05)。且血管活性肠肽表达与软骨改良Mankin病理评分呈负相关(r=-0.896,P<0.05)。说明血管活性肠肽低表达与关节软骨退变程度、骨关节炎病程进展有关,可能是关节软骨退变、骨关节炎发病的机制之一。

BACKGROUND：The discovery of neuropeptide provides new hope for the treatment of osteoarthritis,however,the relationship between neuropeptide expression and pathogenesis of osteoarthritis or degeneration of articular cartilage remains poorly understood.OBJECTIVE：To observe the expression of vasoactive intestinal peptide（VIP） in normal and osteoarthritic chondrocytes,and to explore the correlation between VIP and pathogenesis and progression of osteoarthritis.METHODS：Human cartilages were obtained at arthroplasty from 26 patients with osteoarthritis.Meanwhile,articular cartilages were obtained at arthroplasty or amputation for trauma,tumour or infection from 10 patients with no history of arthritis.Then collect 50 cartilage specimens from osteoarthritis and normal joints,all the specimens were grouped according to Mankin pathological score,and the expression and distribution of VIP was detected by immunohistochemical staining.RESULTS AND CONCLUSION：VIP positive nerve fibers existed in both normal and osteoarthritic cartilages.The expression of VIP was greater in normal cartilage than that of the osteoarthritic cartilages（P〈0.05）.The VIP expression was negative correlated with Mankin pathological scores（r=-0.896,P〈0.05）.The findings demonstrated that,low expression of VIP is concerned with pathogenesis and progression of osteoarthritis,which may be the mechanism for pathogenesis and progression of osteoarthritis.