补气活血类中药黄酮与锌协同的抗自由基作用

Anti-free radical effects of flavone,invigorating qi and promoting blood circulations,synergized with zinc

背景:药物总黄酮的含量与药物的种类有密切的关系,将提取中药中黄酮与微量元素结合,研究药物抗自由基效果,是研究中药药效机制的有效方法。目的:观察补气活血类16种中药黄酮的含量、抗氧化作用,以及与微量元素锌协同作用抗自由基的效果。方法:采用醇提法提取丹参等补气活血类16种中药中的总黄酮,紫外分光光度法测定药物总黄酮的含量,测定不同中药黄酮与锌作用抗氧化率。结果与结论:不同补气活血类中药的总黄酮含量有一定差异,含量依次为是丹参>虎杖>赤芍>泽兰>充玉子>白芍>银杏叶>川芎>牛膝>当归>坤草>回心草>党参>黄芪>人参>郁金,但不同中药黄酮均能够抑制超氧阴离子自由基。与微量金属锌协同作用后,多数中药对超氧阴离子自由基的清除率增加,特别是人参黄铜的抗氧化能力提高效率达7倍,说明补气活血类药物作用可能与金属锌协同相关。

BACKGROUND：Contents of total flavonoids are closely related to species.It is an effective method to research pharmacodynamic mechanism of traditional Chinese medicine by combining Chinese medicine flavone with minor element for anti-free radical.OBJECTIVE：To study the content and antioxidation of flavone in 16 kinds of Chinese medicine,and to explore the effects of flavone synergized with zinc on anti-free radical.METHODS：The contents of total flavones in 16 kinds of traditional Chinese medicine were extracted using alcohol extraction and measured by UV.The oxidation resistance effect of total flavone synergized with zinc on anti-free radical was determined.RESULTS AND CONCLLUSION：There were certain variation of total flavones contents in different kinds of invigorating qi and promoting blood circulation traditional Chinese medicine,the contents as follows：salvia miltiorrhiza giant knotweed red peony root trumpetweed sufficient jade white peony root ginkgo leaf ligusticum chuanxiong hort achyranthes root Chinese angelica motherwort rhodobryum roseum codonopsis pilosula milkvetch root ginseng curcuma longa L.,but all the Chinese medicine could suppress superoxide anion radical.Most of traditional Chinese medicine increased the effect of oxidation resistance after zinc synergism,especially ginseng flavones,which increased 7-fold.The results demonstrated that the drug action of invigorating qi and promoting blood circulation traditional Chinese medicine may relates to zinc synergism.