仙灵骨葆治疗骨质疏松大鼠:血清学及骨组织形态计量学评价

Xianlinggubao for treatment of osteoporotic rats:Serological and bone histomorphometric evaluation

背景:目前对于骨质疏松的治疗多为抑制骨吸收,有研究报道仙灵骨葆可促进骨形成,但具体机制不清。目的:通过干预去卵巢大鼠骨质疏松动物模型,探讨仙灵骨葆对骨质疏松大鼠骨量、生物力学性能及骨代谢的影响。方法:将24只大鼠随机分为正常对照组、模型组和仙灵骨葆组。除正常对照组外,其他2组行卵巢切除。6周后仙灵骨葆组给予仙灵骨葆干预,模型组给予等量双蒸水。4周后各组留取尿液、血清检测血Ⅰ型原胶原氨基端延长肽、尿脱氧吡啶诺啉排泄率和Ⅰ型胶原氨基末端肽排泄率。取各组左侧股骨行骨密度测定,取左侧胫骨制备硬组织不脱钙切片,行骨组织形态计量学检测。结果与结论:与模型组比较,仙灵骨葆组血Ⅰ型原胶原氨基端延长肽、尿脱氧吡啶诺啉排泄率、尿Ⅰ型胶原氨基末端肽排泄率,破骨细胞数、骨吸收周长百分数显著降低(P<0.05),而远端骨密度和骨小梁体积均显著增高(P<0.05),结果证实,仙灵骨葆可抑制骨吸收,促进骨形成,降低去卵巢大鼠骨转换水平,进而部分阻止其骨量丢失。

BACKGROUND：The treatment for osteoporosis mainly targeted on inhibition of bone-resorption at present,Xianlinggubao（XLGB） was reported to be effective for promoting bone formation,but the detail mechanism remains unclearly.OBJECTIVE：To investigate the effects of XLGB on bone mass,biomechanical properties and bone metabolism of osteoporotic rats induced by ovariectomy.METHODS：Twenty-four rats were randomized into 3 groups：normal control group,bilateral ovariectomized group（model）,and XLGB treatment group.Rats received bilateral ovariectomized operation except those in the control group.Six weeks later,rats in XLGB group were administered with XLGB,and in the model group administered with distilled water.Content of procollagen typeⅠN-terminal propeptide（PINP） in blood serum and dihydropyrimidine dehydrogenase/creatinine（DPYD/Cr）,N-telopeptide of typeⅠcollagen/creatinine（NTX/Cr） in urine were detected at 4 weeks after operation,the left femurs were collected for bone mineral density test,and left tibias were prepared for the measurement of bone histomorphometry.RESULTS AND CONCLUSION：Compared with the model group,the levels of PINP,DPYD/Cr,NTX/Cr,number of osteoclasts and absorption percentage（P〈0.05）,but the proximal bone mineral density and bone trabecula volume was dramatically increased（P〈0.05）.The results demonstrated that XLGB could partly prevent bone loss in ovariectomized rats by inhibiting bone resorption and promoting bone formation,thereby reduce the bone turnover level.