增殖细胞核抗原和C-Fos蛋白在人胎胃壁组织中的表达

Expression of proliferating cell nuclear antigen and C-Fos protein in fetal stomach tissues

背景:增殖细胞核抗原表达增高可促进细胞DNA合成增加,反映细胞增殖状况。C-Fos与DNA结合可直接调节具有转录活性的转录因子,在胚胎细胞的发育过程中,对细胞的生长、分化及增殖起促进作用。目的:观察增殖细胞核抗原和C-Fos蛋白在人胎胃组织中的表达。方法:应用免疫组织化学法和图像分析软件检测第2,3,4三个月龄段,人胎胃组织细胞中PCNA和C-Fos蛋白阳性表达的积分吸光度。结果与结论:第2～4个月胎龄段,增殖细胞核抗原和C-Fos蛋白在人胎胃壁各层组织细胞均呈阳性表达。随着胎龄的增大,胃壁组织中增殖细胞核抗原蛋白阳性表达先升高再降低,C-Fos蛋白阳性表达则逐渐增高(P<0.01)。提示增殖细胞核抗原和C-Fos蛋白在人胚胎早期胃组织细胞的生长发育过程中起重要的调节作用。

BACKGROUND：High expression of proliferating cell nuclear antigen（PCNA） can promote cell DNA synthesis and reflect condition of cell proliferation.C-Fos combined with DNA can directly regulate transcription factors,which promote cell growth,differentiation and proliferation during development of embryonic cells.OBJECTIVE：To explore the expression of PCNA and C-Fos proteins in the stomach tissues of human fetus.METHODS：The PCNA and C-Fos positive cells in the stomach tissues of human fetus were tested in the second,third and fourth month of fetal periods by the immunohistochemical methods.And the integral absorbance（IA） was analyzed with Nikon imaging system（NIS-DR）.RESULTS AND CONCLUSION：The positive expressions of PCNA and C-Fos protein were detected in the all of layers of human fetal stomach in the second,third and fourth month of fetal periods.With the fetal age increasing,the expression of IA of PCNA positive cells showed an up-then down-trend,but positive expression of C-Fos protein gradually increased（P〈0.01）.PCNA and C-fos proteins play an important role in regulating the growth and development of human fetal stomach tissues.