放射性咀嚼肌损伤模型软组织病理改变及转化生长因子β1的表达(英文)

Pathological changes of soft tissues and transforming growth factor beta 1 expression in radiation masseter injury models

背景:长期的放射线累积更可引起周围组织纤维化,张口受限,严重影响肿瘤患者的生活品质。目的:观察大鼠咀嚼肌放射损伤后软组织的病理改变及体内转化生长因子β1mRNA的表达。方法:成年Wistar大鼠30只,随机分成对照组(n=10)和放射组(n=20)。放射组利用直线加速器累积照射咀嚼肌40Gy。光镜和电镜下观察咀嚼肌放射损伤区血管病理改变及RT-PCR检测咀嚼肌放射损伤区转化生长因子β1基因表达。结果与结论:放射组咀嚼肌放射损伤区毛细血管密度较对照组明显降低(P<0.01),转化生长因子β1mRNA水平较对照组显著升高(P<0.01)。提示放射损伤可促使组织纤维化修复放射损伤。

BACKGROUND：Long-term radioactive ray accumulation leads to surrounding tissues fibrosis accompanied by limitation of mouth-opening,which seriously affects life quality of tumor patients.OBJECTIVE：To observe pathological change of soft tissues in rats with radiation masseter injury and the level of transforming growth factor β1（TGF-β1） mRNA expression in vivo.METHODS：A total of 30 Wistar rats were randomly divided into control group（n=10） and radiated group（n=20）.Radiated group were radiated with linear accelerator at a dose of 40 Gy.The pathological changes of vessels were observed under light microscope and electron microscope and expression of TGF-β1 was detected by RT-PCR.RESULTS AND CONCLUSION：The microvessel density of radiated group was obviously decreased than that of the control group（P〈0.01）,but the mRNA expression of was higher than that in the control group（P〈0.01）.It suggests that radiated injury can induce fibrosis to repair the radiated injury.