不同浓度淫羊藿含药血清干预骨髓基质干细胞的成脂分化

Adipogenic differentiation of bone marrow stromal stem cells intervened with serum containing different concentration Epimedium

背景:如何抑制骨髓基质干细胞成脂分化,促进其成骨分化将是防治骨质疏松的新途径之一。目的:观察不同浓度淫羊藿含药血清对骨质疏松大鼠骨髓基质干细胞成脂分化的干预作用。方法:全骨髓培养法培养SD大鼠骨髓间充质干细胞,12只SPF级SD雌性大鼠按体表面积的方法给予淫羊藿水提液,药物以蒸馏水定容至所需浓度,相当于临床剂量的20倍、10倍、5倍灌胃,连续灌胃7d后眼眶取血,观察各组干预后7d成脂分化情况。结果与结论:中药低、中剂量含药血清组的成脂率低于空白组(P<0.01、0.05),说明中药淫羊藿含药血清发挥了抑制骨质疏松骨髓基质干细胞向脂肪细胞分化的作用。中药各剂量组的组间比较差异无显著性意义(P>0.05),但3组成脂率数值方面以中剂量组最佳。

BACKGROUND：How to inhibit adipogenic differentiation of bone marrow stromal stem cells（BMSCs） and promote the osteogenic differentiation of BMSCs is a new approach to prevention and treatment of osteoporosis.OBJECTIVE：To investigate the effect of serum containing different concentration Epimedium on adipogenic differentiation of BMSCs in rats with osteoporosis.METHODS：Three of 15 SPF-class SD female rats were used to isolate and culture BMSCs.The remaining 12 rats were divided into control group,high dose herb group,medium dose herb group,low dose herb group.Adipogenic differentiation was observed after 7 day＇s intervention.RESULTS AND CONCLUSION：Medium and low dose herb groups played the effects on suppressing adipogenic differentiation of BMSCs（P0.01,0.05）.There was no significant difference among each herb groups（P0.05）.The medium dose herb was the best in different concentration dose herb on inhibiting adipogenic differentiation of BMSCs.