糖尿病模型皮肤创面中人真皮多能干细胞向表皮细胞的分化

Differentiation of human dermal-derived multipotent stem cells into epidermal cells in diabetic wound

背景：研究表明,干细胞分化为何种细胞与其所处的微环境密切相关。因此设想：人真皮多能干细胞处于皮肤创面的微环境中,可能具有向人皮肤细胞转化的潜能。目的：探讨糖尿病皮肤创面微环境中的人真皮多能干细胞分化为表皮细胞的可能性。方法：分离培养人真皮多能干细胞并制作糖尿病裸鼠模型皮肤创面,将标记5-BrdU的第3~5代人真皮多能干细胞以注射方式回植入糖尿病裸鼠创面组织周围,分别于注射后2,3周取材,常规石蜡包埋、连续切片,行BrdU和角蛋白免疫组织化学染色。结果与结论：BrdU阳性细胞出现在表皮中,且连续切片中部分BrdU阳性细胞也同时表达角蛋白。说明在糖尿病创面愈合过程中,人真皮多能干细胞具有向表皮细胞分化的潜能。

BACKGROUND：Differentiation of stem cells is closely related to the microenvironment.Therefore,it is assumed that human dermal-derived multipotent stem cells may have a potential to differentiate into epidermal cells in skin wound.OBJECTIVE：To explore the feasibility of human dermal-derived multipotent stem cells differentiating into epidermal cells under the microenvironment of diabetic wound.METHODS：Human dermal-derived multipotent stem cells were isolated and cultured.Diabetic naked mouse models were prepared.The third to fifth generation of human dermal-derived multipotent stem cells labeled with 5-bromodeoxyuridine（5-BrdU） were injected into the skin tissue surrounding the wound of diabetic mouse models.The specimens were harvested from the wound tissues for paraffin embedding,and serial sections were made at the 2nd and 3rd week after transplantation for BrdU and keratin staining.RESULTS AND CONCLUSION：Not only BrdU positive cells aggregated in the epidermis but also some positive cells expressed keratin simultaneously in the epidermis.During the course of diabetic wound healing,human dermal-derived multipotent stem cells have the potential to differentiate into epidermal cells.