干细胞诱导分化为肝细胞的鉴定方法

Identification of hepatocytes differentiated from stem cells

背景:目前关于干细胞体外诱导分化为肝细胞的各种方法较多,需要总结一套具有说服力的鉴定方法去验证。目的:总结干细胞经诱导分化为肝细胞鉴定方法的研究进展,使之更安全的应用。方法:应用计算机检索西方生物医学期刊及PubMed数据库1985-01/2010-06有关干细胞分离、培养及诱导分化为肝细胞的文章,检索词为"stemcells,induced,differentiation,hepatocytes,identification"等;同时检索中国期刊全文数据库2000-01/2010-06有关干细胞分离、培养诱导分化为肝细胞的文章,检索词为"干细胞,诱导,分化,肝细胞,鉴定"。共检索到文献43篇。结果与结论:干细胞具有可塑性特性,通过体外刺激因子、体内利用特定的微环境均可诱导干细胞分化为肝前体细胞和成熟肝细胞,理论上可提供丰富的肝细胞来源,为人们提供种子细胞,可以进行实验研究、临床应用。但是经诱导后的细胞是否为所预期的种子细胞需要一套科学的、系统的检测方法,根据肝细胞的诸多特性,如表面标志、合成、代谢功能可以证明。

BACKGROUND：Various methods can induce stem cells differentiate into hepatocytes,so we need a set of convincing method to test.OBJECTIVE：To summarize the identification advances in the methods for testing stem cells differentiate into hepatocytes.METHODS：A computer-based search was conducted in Foreign Medical Journal Full-Test Service and Pubmed for English articles about isolation,cultivation and induction of stem cells published between January 1985 and June 2010 with the key words of ＂stem cells,induction,differentiation,hepatocytes,identification＂.Meanwhile,Chinese Journal Full-text Database was undertaken to identify relevant articles published between January 2000 and June 2010.A total of 43 articles were collected.RESULTS AND CONCLUSION：Stem cells are characterized by plasticity.Stimulating factors in vitro and special microenvironment in vivo could induce stem cells differentiate into hepatocyte precursors and mature hepatocytes.Stem cells can provide a rich source of liver cells and provide seeds cells for us in theory,which can be applied in experiments and clinic.However,whether the induced cells are what we expected needs a set of scientific and systematic detection methods,and we can prove it according to a few characteristics of hepatocytes,such as surface makers,synthetical and metabolic effects.