DNA修复基因MMS19单核苷酸多态性与非小细胞肺癌组织学类型的关系

Relationship between DNA repair gene MMS19 single nucleotide polymorphisms and histology of non-small cell lung cancer

目的:探讨DNA修复基因甲基磺酸敏感性基因19(methyl methanesulfonate sensitivity gene19,MMS19)单核苷酸多态性与非小细胞肺癌(non-small cell lung carcer,NSCLC)组织学类型的关系。方法:收集原发性肺癌患者299例,应用AllGloTM探针结合实时荧光定量-PCR(real-time fluorogentic quantitative-PCR,RFQ-PCR)的方法分析MMS19基因rs3740526多态位点的基因型分布情况,采用卡方和回归方法比较不同基因型与NSCLC患者年龄、性别、是否吸烟以及肺癌组织学类型的关系。结果:MMS19(rs3740526)基因型在不同年龄、性别、吸烟群体中的分布,差异均无统计学意义(P值分别为0.609、0.621和0.755),在不同组织学类型(鳞癌和腺癌)中的分布,差异有统计学意义(P=0.026)。调整后比值比(odds ratio,OR)值分别为0.359[95%可信区间(confi dence interval,CI)=0.162～0.795,P=0.012]和0.445(95%CI=0.213～0.930,P=0.031)。结论:DNA修复基因MMS19rs3740526的多态性可能与NSCLC组织学类型具有相关性。

Objective： To investigate the association between the single nucleotide polymorphisms （SNPs） of methyl methanesulfonate sensitivity gene 19 （MMS19） and the histology of non-small cell lung cancer. Methods： Two hundred and ninety-nine patients with primary non-small cell lung cancer were collected. The MMS19 （rs3740526） genotypes were analyzed by using AllGloTM probe in combination with real-time fluorescence quantitative PCR （RFQ-PCR）. The differences in the frequency distribution of genotypes by age, gender, smoking history and histology were analyzed by chi-square test and logistic regression. Results： There was no difference in the frequency distribution of genotypes by age, gender and smoking history （P=0.609, P=0.621 and P=0.755, respectively）, but there was significant difference by histology （adenocarcinoma vs squamous cell carcinoma, P=0.026）. Adjusted odds ratios （ORs） were 0.359 [95% confidence interval （CI）： 0.162-0.795, P=0.012] and 0.445 （95% CI： 0.213-0.930, P=0.031）. Conclusion： This study confirms that MSS19 （rs3740526） SNPs is associated with the histology of non-small cell lung cancer.