深低温冻储同种异体气管移植并骨髓间充质干细胞移植后气管上皮细胞血管内皮生长因子的表达

Effect of bone marrow derived mesenchymal stem cells transplantation combined with cryopreservation on trachea allograft epithelium vascular endothelial growth factor levels

背景:深低温冷冻后的组织可以保持其原有的活力和组织完整性,并能消减抗原性。目的:静脉移植骨髓间充质干细胞合并深低温处理供体气管后进行同种异体气管移植,观察骨髓间充质干细胞在促进上皮再生和再血管化后的作用。方法:用深低温冻储2周和6周的气管进行Wistar大鼠同种异体气管移植后,用PKH-26标记的3～5代骨髓间充质干细胞经鼠尾静脉移植入受体内,等量的磷酸盐缓冲液注射作为实验对照。观察供体气管的组织学和血管内皮生长因子蛋白表达。结果和结论:骨髓间充质干细胞移植合并深低温冻储后的移植气管结构完整,软骨无变性坏死;深低温6周的移植气管上皮再生情况优于2周。骨髓间充质干细胞移植组的上皮细胞表达血管内皮生长因子水平高于磷酸盐缓冲液对照组。结果提示,骨髓间充质干细胞移植合并深低温冻储6周后移植气管存活期好于深低温冻储2周;骨髓间充质干细胞能够促进移植物周围新生血管的增加,从而促进气管损伤的修复。

BACKGROUND：Cryopreserved tissues can maintain their vitality and integrity, and reduce the antigenicity. OBJECTIVE：Using intravenous transplantation of bone marrow derived mesenchymal stem cells （BMSCs） combined with cryopreserved allograft, to investigate the role of BMSCs on enhancing the implant survival and revascularization by measuring vascular endothelial growth factor （VEGF） levels. METHODS：After transplanted with cryopreserved 2 weeks and 6 weeks allocraft, PKH-26 labeled 3-5 passage BMSCs were injected into the recipient rats via the tail vein. Rats in the control groups were injected with the same amount of phosphate buffer solution （PBS）. We observed the histology of the transplanted trachea and measured the VEGF protein levels in the epithelium to evaluate the role of BMSCs transplantation on the epithelium revascularization. RESULTS AND CONCLUSION：Rats from BMSCs injection group survived a long period. Histological observation showed that the tracheal lumen was covered by psudo-striated ciliated columnar epithelium. VEGF protein levels in the BMSCs injection group were higher than PBS group. BMSCs may exert their reparation function via enhancing epithelium VEGF expression and revascularization.