大鼠胆管缺血狭窄模型的构建

Establishment of a rat model of ischemic biliary strictures induced by clamping

背景:有研究表明可以用钳夹法制作心肌缺血再灌注损伤模型。目的:采用钳夹法建立大鼠胆管缺血狭窄动物模型。方法:将Wistar大鼠随机分为假手术组和模型组,模型组用2枚显微血管夹夹闭一段长约0.8cm的胆总管90min,假手术组仅暴露胆管未夹闭。术后21d两组大鼠均经静脉胆道造影,下腔静脉取血,分离血清进行总胆红素及直接胆红素浓度测定。结果与结论:模型组钳夹部位以上胆管明显扩张,静脉胆管造影示胆管扩张,壁光滑,狭窄部位以下胆管未显影。3只(16.7%)胆道未显影,但解剖动物时发现其肝脏肿大,微呈绿色,钳夹处胆总管闭锁,胆总管扩张。模型组血清总胆红素及直接胆红素高于假手术组(P<0.05)。结果证实,实验采用血管夹钳闭缺血法成功构建了大鼠胆管缺血狭窄模型。

BACKGROUND：Studies have showed that forceps operation can be used to prepare myocardial ischemia-reperfusion injury models. OBJECTIVE：To prepare a rat biliary ischemic stenosis model using forceps operation. METHODS：After the optimal time of biliary ischemia was determined, 40 Wistar rats were randomly divided into 2 groups. 0.8 cm common bile duct was clamped for 90 minutes with 2 micro-vessel clips in the experimental group. The common bile duct was not clamped in the sham operation group. Twenty-one days later, the intravenous cholangiography was conducted in all the rats. Blood samples were collected from the inferior vena cava to investigate the serum concentration of total bilerubin （TBIL） and direct bilerubin （DBIL）. RESULTS AND CONCLUSION：Twenty-one days later：3 rats in experimental group could be evidenced to bile duct distension by intravenous cholangiography. But they all had serious dilatation, and their liver was bad when autopsy was done. Obvious dilatation of the common bile duct appeared over the clamped site. There was no dilatation of the common bile duct in the sham operation group. The serum concentration of TBIL and DBIL were significantly higher in the experimental group than in the sham operation group （P 0.05）. The result proved that The rat model of ischemic biliary strictures resulting from clamping has been successfully established.