原发性肝癌组织标本采集、保存数据库及组织库的建立与管理

Establishment and management of hepatocarcinoma tissue bank and data base

背景:目前,肝癌的遗传资源并没有得到很好的收集和保护,为了保存广东省宝贵的肝癌家系,南方医科大学附属珠江医院建立了肝癌组织库及数据库。目的:探讨肝癌组织标本的采集和保存以及组织库的建立和信息化管理方法。方法:收集手术切除的肿瘤组织、癌旁组织、远端正常组织及患者的血清、血浆以及从肿瘤组织中提取的核酸,分别保存组织块、核酸、血清、血浆、淋巴细胞、干细胞等,液氮或-80℃冷冻保存;同时建立一套信息系统用于肿瘤组织库的管理,包括标本存放位置、标本使用、实验结果、综合查询、标本废除管理等。结果与结论:收集标本45例,其中良性肿瘤5例,恶性肿瘤40例,共350份标本,1年来肿瘤组织库运转良好。建立肝癌组织库及数据库可以对标本进行质量控制及信息化管理,保存少见、罕见标本,达到资源保存和共享的目的。

BACKGROUND：Currently, the genetic resource of hepatocarcinoma has not be collected and protected well;therefore, in order to save the proband pedigrees of Guangdong Province, a information-based management of tumor database was established in Zhujiang Hospital Affiliated to Southern Medical University. OBJECTIVE：To investigate the collection and conservation of tumor tissue specimens, and the approaches to information-based management of tumor database. METHODS：The tumor tissues, tumor-adjacent tissues, distal normal tissues removed from surgery and patient serum and extracted nucleic acids from tumor tissues were collected. Specimens of the tissue fragments, nucleic acids, serum, plasma, lymphocyte and stem cells, etc. were respectively conserved in liquid nitrogen or -80 ℃ refrigerator. Meanwhile, a database of information management of tumor tissue bank was established, including positions of sample placement, specimen use, laboratory results, comprehensive query, management of specimen disposal, etc. RESULTS AND CONCLUSION：Specimens of 45 cases, including relevant specimens of 5 cases of benign tumors and 40 cases of malignant tumors, were collected. The tumor tissue data base was established and managed according to the above-mentioned methods, which functioned with good results in the past 1 year. The establishment of tumor tissue database enables the quantity control of the specimens and information-based management, as well as conservation of infrequent specimens and resource share.