鸡传染性贫血免疫对肝微粒体抗氧化酶活性的影响

Effects of vaccinating chickens against chicken infectious anemia immunity on the antioxidant enzyme activity in liver microsomes of chickens

目的是观察鸡传染性贫血多次免疫对肝微粒体中抗氧化酶活性的影响。对20只SPF鸡随机分为2组,每组10只,免疫组鸡用鸡传染性贫血弱毒苗免疫4次,每次间隔2周,对照组鸡注射同剂量的生理盐水。最后一次免疫后10 d取肝脏制备微粒体,利用测试盒测定肝微粒体中的GSH-PX活性、SOD活性、CAT活性和MDA含量。结果与对照组相比,免疫组肝微粒体中GSH-PX活性、SOD活性和CAT活性都显著提高(P<0.05),MDA含量显著减低(P<0.05)。结论为鸡传染性贫血多次免疫可提高鸡体抗氧化能力。

To investigate the effect of vaccinating chickens against chicken infectious anemia（chicken infectious anemia,CIA） on the antioxidant enzyme activity in liver microsomes of chickens,20 chickens were randomly divided into two groups with 10 chickens each.The chickens in immunized group were vaccinated with CIA attenuated vaccine 4 times at an interval of 2 weeks.The chickens in control group were injected by NS.The liver microsomes were prepared 10 d after the last immunization.GSH-PX activity,SOD activity,CAT activity and MDA contention in liver microsomes were determined by test-box.Compared with the control group,GSH-PX activity,SOD activity and CAT activity in liver microsomes significantly improved in immunization group（P0.05）.MDA contents in liver microsomes wres significantly reduced in immunization group（P0.05）.The conclusion is that the antioxidant capacity are increased by multiple CIA several immunizations.