膀胱尿路上皮癌组织中Snail蛋白表达与E—cadherin蛋白、T细胞亚群的相关性分析

Expression of Snail in bladder urotheliai carcinoma and its relationship with E-cadherin and a subset of T cell Groups

目的研究膀胱尿路上皮癌组织中Snail蛋白表达与E—cadherin蛋白、T细胞亚群的相关性。方法采用免疫组化SP法检测156例膀胱尿路上皮癌组织和80例癌旁组织中Snail蛋白、E．cadherin蛋白的表达情况，比较二者在不同病理组织中的阳性表达率，并分析二者表达的相关性；分析膀胱尿路上皮癌组织中Snail蛋白阳性表达和CD4^＋、CD8^＋细胞数量及CD4^＋／CD8^＋的相关性。结果膀胱尿路上皮癌组织Snail蛋白阳性表达率65．4％（102／156）显著高于癌旁组织的48．8％（39／80），差异有统计学意义（P〈0．05）；其表达与膀胱尿路上皮癌的临床分期、病理分级、肿瘤数量、远处转移及复发有关（P值均〈0．05）。膀胱尿路上皮癌中Snail蛋白与E—cadherin蛋白表达呈负相关（r=-0．186，P〈0．05）；Snail蛋白阳性表达与CD4^＋细胞数量以及CD4^＋／CD8^＋值呈负相关（r=-0．313，P〈0．05；r=-0．305，P〈0．05），而与CD8^＋细胞数量无关（r=-0．250，P〉0．05）。结论Snail蛋白可能通过抑制E—cadherin蛋白表达及诱导膀胱肿瘤局部免疫抑制，促进膀胱尿路上皮癌的浸润、转移。

Objective To investigate the expression of Snail in bladder urothelial carcinoma and evaluate its relationship with E-cadherin and a subset of T cell groups. Methods Immunohistochemical method was used to detect the expression of Snail and E-cadherin proteins in tissue from 156 cases of bladder urothelial carcinoma and 80 cases of the para-cancerous mucosa. The proteins expression status was analyzed with clinico-pathological data. Meanwhile, correlations with Snail and CD4^＋, CD8^＋ , CD4^＋/CDs were analyzed. Results The positive rate of Snail in bladder urothelial carcinoma was 65.4% , which was significantly higher than that in para-cancerous mucosa （48.8%）. The expression of Snail was significantly correlated with the clinical stage, pathological grade, tumor quantity, distant metastasis and tumor recurrence. There was negative correlation between the expression of Snail and E-cadherin in bladder urothelial carcinoma. Positive expression of Snail had a negative correlation with the number of CD4^＋ and CD4^＋/CD8^＋ , whereas it had no significant correlation with the number of CDs. Conclusions By inhibition of the E-cadherin expression and inducing local immunosuppression, Snail might play an important role in the process of the invasion and metastasis of bladder urothelial carcinoma.